ID   BUB32_ARATH             Reviewed;         339 AA.
AC   Q9C701; Q9C6C4;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Mitotic checkpoint protein BUB3.2;
DE   AltName: Full=Protein BUDDING UNINHIBITED BY BENZYMIDAZOL 3.2;
GN   Name=BUB3.2; OrderedLocusNames=At1g49910; ORFNames=F10F5.12, T18C15.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RX   PubMed=18508582; DOI=10.2741/3076;
RA   Lermontova I., Fuchs J., Schubert I.;
RT   "The Arabidopsis checkpoint protein Bub3.1 is essential for gametophyte
RT   development.";
RL   Front. Biosci. 13:5202-5211(2008).
CC   -!- FUNCTION: Has a dual function in spindle-assembly checkpoint signaling
CC       and in promoting the establishment of correct kinetochore-microtubule
CC       (K-MT) attachments. Promotes the formation of stable end-on bipolar
CC       attachments. Necessary for kinetochore localization of BUB1. The
CC       BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting
CC       complex or cyclosome (APC/C) when spindle-assembly checkpoint is
CC       activated and inhibits the ubiquitin ligase activity of APC/C by
CC       phosphorylating its activator CDC20 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the mitotic checkpoint complex (MCC). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Cytoplasm, cytoskeleton, phragmoplast
CC       {ECO:0000250}. Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC       Note=Accumulates onto both kinetochores and the spindle microtubules in
CC       cell arrested in metaphase. Starts to localize at kinetochores in
CC       prometaphase I (Pro-MI) stage and maintains the localization until the
CC       metaphase I-anaphase I (MI-AI) transition. Associates with unattached
CC       kinetochores upon spindle assembly checkpoint (SAC) activation. Present
CC       in the phragmoplast midline during the final step of cell division (By
CC       similarity). {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in actively dividing tissues such as
CC       roots, flower buds, flowers and siliques.
CC       {ECO:0000269|PubMed:18508582}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC       {ECO:0000269|PubMed:18508582}.
CC   -!- SIMILARITY: Belongs to the WD repeat BUB3 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51772.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC074110; AAG60165.1; -; Genomic_DNA.
DR   EMBL; AC079674; AAG51772.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE32490.1; -; Genomic_DNA.
DR   RefSeq; NP_175413.1; NM_103878.2.
DR   AlphaFoldDB; Q9C701; -.
DR   SMR; Q9C701; -.
DR   STRING; 3702.AT1G49910.1; -.
DR   PaxDb; Q9C701; -.
DR   PRIDE; Q9C701; -.
DR   ProteomicsDB; 239105; -.
DR   EnsemblPlants; AT1G49910.1; AT1G49910.1; AT1G49910.
DR   GeneID; 841414; -.
DR   Gramene; AT1G49910.1; AT1G49910.1; AT1G49910.
DR   KEGG; ath:AT1G49910; -.
DR   Araport; AT1G49910; -.
DR   TAIR; locus:2007248; AT1G49910.
DR   eggNOG; KOG1036; Eukaryota.
DR   HOGENOM; CLU_038526_0_0_1; -.
DR   InParanoid; Q9C701; -.
DR   OMA; EFKHGGA; -.
DR   OrthoDB; 1048963at2759; -.
DR   PhylomeDB; Q9C701; -.
DR   PRO; PR:Q9C701; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C701; baseline and differential.
DR   Genevisible; Q9C701; AT.
DR   GO; GO:1990298; C:bub1-bub3 complex; IBA:GO_Central.
DR   GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR   GO; GO:0033597; C:mitotic checkpoint complex; IBA:GO_Central.
DR   GO; GO:0009524; C:phragmoplast; IBA:GO_Central.
DR   GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 3.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 5.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Cytoplasm; Cytoskeleton; Kinetochore; Meiosis; Mitosis; Nucleus;
KW   Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..339
FT                   /note="Mitotic checkpoint protein BUB3.2"
FT                   /id="PRO_0000423381"
FT   REPEAT          14..53
FT                   /note="WD 1"
FT   REPEAT          95..134
FT                   /note="WD 2"
FT   REPEAT          141..178
FT                   /note="WD 3"
FT   REPEAT          238..277
FT                   /note="WD 4"
FT   REPEAT          280..323
FT                   /note="WD 5"
SQ   SEQUENCE   339 AA;  37670 MW;  7BF63729AF01B8B2 CRC64;
     MTLVPAIGRE LSNPPSDGIS NLRFSNNSDH LLVSSWDKSV RLYDANGDLM RGEFKHGGAV
     LDCCFHDDSS GFSVCADTKV RRIDFNAGKE DVLGTHEKPV RCVEYSYAAG QVITGSWDKT
     IKCWDPRGAS GTERTQIGTY MQPERVNSLS LVGNRLVVAT AGRHVNIYDL RNMSQPEQRR
     ESSLKYQTRC VRCYPNGTGY ALSSVEGRVS MEFFDLSEAA QAKKYAFKCH RKSEDGRDIV
     YPVNAIAFHP IYGTFASGGC DGFVNIWDGN NKKRLYQYSK YPTSIAALSF SRDGGLLAVA
     SSYTFEEGDK PHEPDAIFVR SVNEIEVKPK PKVYPNPPV