ID   TRAF_AGRFC              Reviewed;         176 AA.
AC   Q44350;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Conjugal transfer protein TraF;
DE   Flags: Precursor;
GN   Name=traF; OrderedLocusNames=Atu6128; ORFNames=AGR_pTi_240;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8763953; DOI=10.1128/jb.178.14.4233-4247.1996;
RA   Farrand S.K., Hwang I., Cook D.M.;
RT   "The tra region of the nopaline-type Ti plasmid is a chimera with elements
RT   related to the transfer systems of RSF1010, RP4, and F.";
RL   J. Bacteriol. 178:4233-4247(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Involved in conjugal transfer of the plasmid. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S26C family. {ECO:0000305}.
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DR   EMBL; AF010180; AAC17207.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK91092.1; -; Genomic_DNA.
DR   PIR; AF3243; AF3243.
DR   PIR; T03420; T03420.
DR   RefSeq; NP_396651.1; NC_003065.3.
DR   RefSeq; WP_010974897.1; NZ_AF010180.1.
DR   AlphaFoldDB; Q44350; -.
DR   MEROPS; S26.014; -.
DR   EnsemblBacteria; AAK91092; AAK91092; Atu6128.
DR   KEGG; atu:Atu6128; -.
DR   PATRIC; fig|176299.10.peg.5335; -.
DR   HOGENOM; CLU_104604_3_0_5; -.
DR   OMA; CPGDYGY; -.
DR   PhylomeDB; Q44350; -.
DR   BioCyc; AGRO:ATU6128-MON; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR019533; Peptidase_S26.
DR   InterPro; IPR014139; Peptidase_S26C_TraF.
DR   Pfam; PF10502; Peptidase_S26; 1.
DR   SUPFAM; SSF51306; SSF51306; 1.
DR   TIGRFAMs; TIGR02771; TraF_Ti; 1.
PE   3: Inferred from homology;
KW   Conjugation; Periplasm; Plasmid; Reference proteome; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..176
FT                   /note="Conjugal transfer protein TraF"
FT                   /id="PRO_0000022577"
SQ   SEQUENCE   176 AA;  18855 MW;  0188B0E5DC8D3B3D CRC64;
     MSKRAVIRFL GVAGLVLSGA TVMGLIGGYR VNVTPSEPLG LWRVEKLRRP VQSGDLVFVC
     PPHNAFFEEA WLRGYLRSGL CPGWFAPLIK SVLALPGQRV EIADRIVIDG HPVSASTVSA
     TDSEGRAIAP FAGGVVPPGF LFLHSSFASS YDSRYFGPIP DSGLLGLARP VFTFDP