ID   TRAF_ECOLI              Reviewed;         247 AA.
AC   P14497;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Protein TraF;
DE   Flags: Precursor;
GN   Name=traF; OrderedLocusNames=ECOK12F091;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=K12;
RX   PubMed=3042757; DOI=10.1128/jb.170.8.3633-3639.1988;
RA   Wu J.H., Kathir P., Ippen-Ihler K.;
RT   "The product of the F plasmid transfer operon gene, traF, is a periplasmic
RT   protein.";
RL   J. Bacteriol. 170:3633-3639(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22, AND SUBCELLULAR LOCATION.
RC   STRAIN=K12; PLASMID=F;
RX   PubMed=1593622; DOI=10.1016/0022-2836(92)90923-8;
RA   Maneewannakul S., Kathir P., Ippen-Ihler K.;
RT   "Characterization of the F plasmid mating aggregation gene traN and of a
RT   new F transfer region locus trbE.";
RL   J. Mol. Biol. 225:299-311(1992).
CC   -!- FUNCTION: Involved in F pilus assembly.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:3042757,
CC       ECO:0000305|PubMed:1593622}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA97961.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; M20787; AAC63064.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44183.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97961.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X61575; CAA43777.1; -; Genomic_DNA.
DR   PIR; A31099; QUECTF.
DR   RefSeq; NP_061470.1; NC_002483.1.
DR   RefSeq; WP_001030367.1; NZ_CP014273.1.
DR   RefSeq; YP_009060141.1; NC_024956.1.
DR   RefSeq; YP_009068333.1; NC_025139.1.
DR   RefSeq; YP_009070598.1; NC_025175.1.
DR   AlphaFoldDB; P14497; -.
DR   SMR; P14497; -.
DR   DIP; DIP-28100N; -.
DR   PRIDE; P14497; -.
DR   PATRIC; fig|83333.107.peg.621; -.
DR   PRO; PR:P14497; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   InterPro; IPR014110; TraF.
DR   InterPro; IPR039555; TraF/TrbB.
DR   Pfam; PF13728; TraF; 1.
DR   TIGRFAMs; TIGR02739; TraF; 1.
PE   3: Inferred from homology;
KW   Conjugation; Periplasm; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..247
FT                   /note="Protein TraF"
FT                   /id="PRO_0000024500"
SQ   SEQUENCE   247 AA;  27973 MW;  78D85B03CFC933F2 CRC64;
     MNKALLPLLL CCFIFPASGK DAGWQWYNEK INPKEKENKP VPAAPRQEPD IMQKLAALQT
     ATKRALYEAI LYPGVDNFVK YFRLQNYWAQ QAGLFTMSAR KAMLAHPELD YNLQYSHYNG
     TVRNQLAADQ AQQRQAIAKL AEHYGIMFFY RGQDPIDGQL AQVINGFRDT YGLSVIPVSV
     DGVINPLLPD SRTDQGQAQR LGVKYFPAMM LVDPKQGSVR PLSYGFISQD DLAKQFLNVS
     EDFKPNF