ID   TRAF_PENCR              Reviewed;         482 AA.
AC   A0A481WQD3;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   03-AUG-2022, entry version 11.
DE   RecName: Full=MFS-type transporter traF {ECO:0000303|PubMed:30811183};
DE   AltName: Full=Terrestric acid biosynthesis cluster protein F {ECO:0000303|PubMed:30811183};
GN   Name=traF {ECO:0000303|PubMed:30811183};
OS   Penicillium crustosum (Blue mold fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=36656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PRB-2;
RX   PubMed=30811183; DOI=10.1021/jacs.9b00110;
RA   Fan J., Liao G., Kindinger F., Ludwig-Radtke L., Yin W.B., Li S.M.;
RT   "Peniphenone and penilactone formation in Penicillium crustosum via 1,4-
RT   Michael additions of ortho-quinone methide from hydroxyclavatol to gamma-
RT   butyrolactones from Crustosic Acid.";
RL   J. Am. Chem. Soc. 141:4225-4229(2019).
CC   -!- FUNCTION: MFS-type transporter; part of the tra gene cluster that
CC       produces terrestric acid (PubMed:30811183). The clavatol biosynthesis
CC       cluster cla and the terrestric acid cluster tra are both involved in
CC       the production of peniphenones and penilactones (PubMed:30811183).
CC       {ECO:0000269|PubMed:30811183}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Does not result in significant changes in
CC       sencondary metabolites production. {ECO:0000269|PubMed:30811183}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. CAR1 family.
CC       {ECO:0000255}.
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DR   EMBL; MK360919; QBK15054.1; -; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..482
FT                   /note="MFS-type transporter traF"
FT                   /id="PRO_0000455058"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        354..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        427..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        448..468
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        15
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        45
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        376
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   482 AA;  52532 MW;  A9FD9760B4797C5E CRC64;
     MTSGTEQATL NTEENGSDSD HLSKEPIAMP ALDWDGPTDS NNPRNFSTPK KVFITACLAS
     LVCISTFGSS VMSPASGQLI HEFGISKELS ILATALYVLG FAVGPLLFGP ASEVLGRKYP
     LSVGVFLFAI FSIPIAVAKN VATIFVCRFL CGTFAAAPLA IAGGGLADLW EPLSRGIAVA
     GFASATFLGP VLGPLVGGFV TKSHLGWRWT QWLSIIFSLV FLAIYFVFCP ETYSPIVLAR
     LAKKRGQMPP GGKVDFKQLA KVYMLRPFIM LVQEPILALL TLYMGFIYGF LYLCFEAFPI
     AFEEHRGWDI GIGSLPFLSI TVGVLIGVVI IIVHTMTRMR RKLETVGDAP EERLVPMMIG
     SILMPAGIFW FAWTSNTSLP WAPQVVSGVF IGCGILLIFL QGMNYIIDVY KVMANSAISI
     NAMFRGLLGA GFPLFASYMF DNLGVPWAMS LLGFLCVALV PVPFLFFIYG ERIRKWSKFT
     AH