ID   TRAG1_ECOLI             Reviewed;         938 AA.
AC   P33790;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Protein TraG;
DE   Contains:
DE     RecName: Full=Protein TraG*;
GN   Name=traG; OrderedLocusNames=ECOK12F099;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=1348105; DOI=10.1007/bf00299147;
RA   Firth N., Skurray R.A.;
RT   "Characterization of the F plasmid bifunctional conjugation gene, traG.";
RL   Mol. Gen. Genet. 232:145-153(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a crucial role in donor-recipient cell interactions.
CC       Required for two stages of the conjugation process: pilus biosynthesis
CC       and mating aggregate stabilization. May interact with TraN.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- PTM: TraG* probably arises from the post-translational cleavage of
CC       TraG.
CC   -!- CAUTION: TraG is not responsible for the N-terminal acetylation of F
CC       pilin as stated by some authors. {ECO:0000305}.
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DR   EMBL; M59763; AAA98081.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44184.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97969.1; -; Genomic_DNA.
DR   PIR; S20480; S20480.
DR   RefSeq; NP_061478.1; NC_002483.1.
DR   RefSeq; WP_001007055.1; NZ_CP014273.1.
DR   AlphaFoldDB; P33790; -.
DR   PRIDE; P33790; -.
DR   PATRIC; fig|83333.107.peg.613; -.
DR   PRO; PR:P33790; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR012931; TraG_N_Proteobacteria.
DR   Pfam; PF07916; TraG_N; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; Conjugation; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..938
FT                   /note="Protein TraG"
FT                   /id="PRO_0000024501"
FT   CHAIN           ?..938
FT                   /note="Protein TraG*"
FT                   /id="PRO_0000024502"
FT   TOPO_DOM        1..53
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..329
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        351..412
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..433
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        434..938
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          654..677
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          915..938
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   938 AA;  102472 MW;  9E09C8402ACDFDDF CRC64;
     MNEVYVIAGG EWLRNNLNAI AAFMGTWTWD SIEKIALTLS VLAVAVMWVQ RHNVMDLLGW
     VAVFVLISLL VNVRTSVQII DNSDLVKVHR VDNVPVGLAM PLSLTTRIGH AMVASYEMIF
     TQPDSVTYSK TGMLFGANLI VKSTDFLSRN PEIINLFQDY VQNCVLGDIY LNHKYTLEDL
     MASADPYTLI FSRPSPLRGV YDNNNNFITC KDASVTLKDR LNLDTKTGGK TWHYYVQQIF
     GGRPDPDLLF RQLVSDSYSY FYGSSQSASQ IMRQNVTMNA LKEGITSNAA RNGDTASLVS
     LATTSSMEKQ RLAHVSIGHV TMRNLPMVQT ILTGIAIGIF PLLILAAVFN KLTLSVLKGY
     VFALMWLQTW PLLYAILNSA MTFYAKQNGA PVVLSELSQI QLKYSNLAST AGYLSAMIPP
     LSWMMVKGLG AGFSSVYSHF ASSSISPTAS AAGSVVDGNY SYGNMQTENV NGFSWSTNST
     TSFGQMMYQT GSGATATQTR DGNMVMDASG AMSRLPVGIN ATRQIAAAQQ EMAREASNRA
     ESALHGFSSS IASAWNTLSQ FGSNRGSSDS VTGGADSTMS AQDSMMASRM RSAVESYAKA
     HNISNEQATR ELASRSTNAS LGLYGDAYAK GHLGISVLGN GGGVGLQAGA KASIDGSDLD
     SHEASSGSRA SHDARHDIDA RATQDFKEAS DYFTSRKVSE SGSHTDNNAD SRVDQLSAAL
     NSAKQSYDQY TTNMTRSHEY AEMASRTESM SGQMSEDLSQ QFAQYVMKNA PQDVEAILTN
     TSSPEIAERR RAMAWSFVQE QVQPGVDNTW RESRRDIGKG MESVPSGGGS QDIIADHQGH
     QAIIEQRTQD SNIRNDVKHQ VDNMVTEYRG NIGDTQNSIR GEENIVKGQY SELQNHHKTE
     ALTQNNKYNE EKLAQERIPG ADSPKELLEK AKSYQHKE