ID   TRAG4_ECOLX             Reviewed;         635 AA.
AC   Q00185;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Conjugal transfer protein TraG;
GN   Name=traG;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-8.
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1665997; DOI=10.3109/10425179109020786;
RA   Ziegelin G., Pansegrau W., Strack B., Balzer D., Kroeger M., Kruft V.,
RA   Lanka E.;
RT   "Nucleotide sequence and organization of genes flanking the transfer origin
RT   of promiscuous plasmid RP4.";
RL   DNA Seq. 1:303-327(1991).
RN   [2]
RP   MUTAGENESIS OF LYS-187; LYS-209; GLU-211; ASP-449 AND LYS-456.
RX   PubMed=8021214; DOI=10.1128/jb.176.14.4285-4295.1994;
RA   Balzer D., Pansegrau W., Lanka E.;
RT   "Essential motifs of relaxase (TraI) and TraG proteins involved in
RT   conjugative transfer of plasmid RP4.";
RL   J. Bacteriol. 176:4285-4295(1994).
RN   [3]
RP   CHARACTERIZATION, AND TOPOLOGY.
RX   PubMed=11976307; DOI=10.1128/jb.184.10.2767-2779.2002;
RA   Schroeder G., Krause S., Zechner E.L., Traxler B., Yeo H.-J., Lurz R.,
RA   Waksman G., Lanka E.;
RT   "TraG-like proteins of DNA transfer systems and of the Helicobacter pylori
RT   type IV secretion system: inner membrane gate for exported substrates?";
RL   J. Bacteriol. 184:2767-2779(2002).
CC   -!- FUNCTION: Required for conjugative transfer of plasmid RP4. Binds
CC       tightly and specifically to the relaxase TraI. Can also bind to DNA
CC       without sequence specificity. May form a pore-like structure that could
CC       serve as a channel for DNA transfer.
CC   -!- SUBUNIT: May form multimers of at least 18 subunits.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
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DR   EMBL; X54459; CAA38334.1; -; Genomic_DNA.
DR   PIR; S22999; S22999.
DR   RefSeq; WP_011205817.1; NZ_NJTR01000055.1.
DR   AlphaFoldDB; Q00185; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Conjugation; Direct protein sequencing;
KW   DNA-binding; Membrane; Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..635
FT                   /note="Conjugal transfer protein TraG"
FT                   /id="PRO_0000221657"
FT   TOPO_DOM        1..22
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..82
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        101..635
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         187
FT                   /note="K->T: Loss of transfer activity."
FT                   /evidence="ECO:0000269|PubMed:8021214"
FT   MUTAGEN         209
FT                   /note="K->T: Decrease in transfer activity."
FT                   /evidence="ECO:0000269|PubMed:8021214"
FT   MUTAGEN         211
FT                   /note="E->Q: Decrease in transfer activity."
FT                   /evidence="ECO:0000269|PubMed:8021214"
FT   MUTAGEN         449
FT                   /note="D->N: Loss of transfer activity."
FT                   /evidence="ECO:0000269|PubMed:8021214"
FT   MUTAGEN         456
FT                   /note="K->T: No change in transfer activity."
FT                   /evidence="ECO:0000269|PubMed:8021214"
SQ   SEQUENCE   635 AA;  69858 MW;  C5AABAF2F9A6F7EC CRC64;
     MKNRNNAVGP QIRAKKPKAS KTVPILAGLS LGAGLQTATQ YFAHSFQYQA GLGWNINHVY
     TPWSILQWAG KWYGQYPDDF MRAASMGMVV STVGLLGTAV TQMVKANTGK ANDYLHGSAR
     WADKKDIQAA GLLPRPRTVV ELVSGKHPPT SSGVYVGGWQ DKDGKFHYLR HNGPEHVLTY
     APTRSGKGVG LVVPTLLSWA HSAVITDLKG ELWALTAGWR KKHARNKVVR FEPASAQGSA
     CWNPLDEIRL GTEYEVGDVQ NLATLIVDPD GKGLESHWQK TSQALLVGVI LHALYKAKNE
     GTPATLPSVD GMLADPNRDV GELWMEMTTY GHVDGQNHPA VGSAARDMMD RPEEESGSVL
     STAKSYLALY RDPVVARNVS KSDFRIKQLM HHDDPVSLFI VTQPNDKARL RPLVRVMVNM
     IVRLLADKMD FENGRPVAHY KHRLLMMLDE FPSLGKLEIL QESLAFVAGY GIKCYLICQD
     INQLKSRETG YGHDESITSN CHVQNAYPPN RVETAEHLSK LTGTTTIVKE QITTSGRRTS
     ALLGNVSRTF QEVQRPLLTP DECLRMPGPK KSADGSIEEA GDMVVYVAGY PAIYGKQPLY
     FKDPIFQARA AVPAPKVSDK LIQTATVEEG EGITI