ID   TRAG5_ECOLX             Reviewed;         637 AA.
AC   Q00184;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Conjugal transfer protein TraG;
GN   Name=traG;
OS   Escherichia coli.
OG   Plasmid IncP-beta R751.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1665997; DOI=10.3109/10425179109020786;
RA   Ziegelin G., Pansegrau W., Strack B., Balzer D., Kroeger M., Kruft V.,
RA   Lanka E.;
RT   "Nucleotide sequence and organization of genes flanking the transfer origin
RT   of promiscuous plasmid RP4.";
RL   DNA Seq. 1:303-327(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Thomas C.M.;
RL   Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for conjugative transfer of plasmid R751. Binds
CC       tightly and specifically to the relaxase TraI. Can also bind to DNA
CC       without sequence specificity. May form a pore-like structure that could
CC       serve as a channel for DNA transfer (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: May form multimers of at least 18 subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X54458; CAA38327.1; -; Genomic_DNA.
DR   EMBL; U67194; AAC64474.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q00184; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Conjugation; DNA-binding; Membrane;
KW   Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..637
FT                   /note="Conjugal transfer protein TraG"
FT                   /id="PRO_0000221658"
FT   TOPO_DOM        1..22
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        44..84
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        106..637
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   637 AA;  69883 MW;  7B45A9A9020902FB CRC64;
     MKIKMNNAVG PQVRTAKPKP SKLLPVLGAA SMVGGLQAAT QFFAHTFAYH ATLGPNVGHV
     YAPWSILHWT YKWYSQYPDE IMKAGSMGML VSTVGLLGVA VAKVVTSNSS KANEYLHGSA
     RWAEKKDIQA AGLLPRERNV LEIVTGKAAP TATGVYVGGW QDKDGNFFYL RHSGPEHVLT
     YAPTRSGKGV GLVVPTLLSW GASSVITDLK GELWALTAGW RQKHAKNKVL RFEPASTSGG
     VCWNPLDEIR LGTEYEVGDV QNLATLIVDP DGKGLDSHWQ KTAFALLVGV ILHALYKAKD
     DGGTATLPSV DAMLADPNRD IGELWMEMAT YGHVDGQNHH AIGSAARDMM DRPEEEAGSV
     LSTAKSYLAL YRDPVVARNV SRSDFRIKQL MHEDDPVSLY IVTQPNDKAR LRPLVRVMVN
     MIVRLLADKM DFEGGRPVAH YKHRLLMMLD EFPSLGKLEI MQESLAFVAG YGIKCYLICQ
     DINQLKSRET GYGHDESITS NCHVQNAYPP NRVETAEHLS RLTGQTTVVK EQITTSGRRT
     AAMLGQVSRT YQEVQRPLLT PDECLRMPGP KKNAQGEIEE AGDMVIYVAG YPAIYGKQPL
     YFKDPVFSAR AAIPAPKVSD RLRAVAQADT EGEGITI