ID   TRAG_AGRFC              Reviewed;         658 AA.
AC   Q44346;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Conjugal transfer protein TraG;
GN   Name=traG; OrderedLocusNames=Atu6124; ORFNames=AGR_pTi_bx12;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8763953; DOI=10.1128/jb.178.14.4233-4247.1996;
RA   Farrand S.K., Hwang I., Cook D.M.;
RT   "The tra region of the nopaline-type Ti plasmid is a chimera with elements
RT   related to the transfer systems of RSF1010, RP4, and F.";
RL   J. Bacteriol. 178:4233-4247(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
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DR   EMBL; AF010180; AAC17204.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK91088.1; -; Genomic_DNA.
DR   PIR; AB3243; AB3243.
DR   PIR; T03416; T03416.
DR   RefSeq; NP_396647.1; NC_003065.3.
DR   RefSeq; WP_010974894.1; NZ_AF010180.1.
DR   AlphaFoldDB; Q44346; -.
DR   EnsemblBacteria; AAK91088; AAK91088; Atu6124.
DR   KEGG; atu:Atu6124; -.
DR   PATRIC; fig|176299.10.peg.5331; -.
DR   HOGENOM; CLU_028732_0_0_5; -.
DR   OMA; ASWIMSD; -.
DR   PhylomeDB; Q44346; -.
DR   BioCyc; AGRO:ATU6124-MON; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014135; Ti-typ_conjug_TS_TraG-like.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02767; TraG-Ti; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Conjugation; Membrane; Plasmid; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..658
FT                   /note="Conjugal transfer protein TraG"
FT                   /id="PRO_0000221654"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          562..585
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          637..658
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        562..583
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   658 AA;  71106 MW;  712D0AC4832A0E2C CRC64;
     MTMNRLLLLI LPAIIMLAAM FATSGMEQRL AAFGTSPQAK LMLGRAGLAL PYIAAVAIGI
     IGLFAANGSA NIKAAGLSVL AGSGVVITIA TLREVIRLNS IASSVPAEQS VLAYADPVTM
     IGASIAFISG MFALRVAIKG NAAFATTAPK RIGGKRAVHG EADWMKIQEA AKLFPERGGI
     IIGERYRVDR DSVAAMPFRA DDRQSWGAGG KSPLLCFDGS FGSSHGIVFA GSGGFKTTSV
     TIPTALKWGG GLVVLDPSSE VAPMVCEHRR QAGRKVIVLD PTAGGVGFNA LDWIGRHGNT
     KEEDIVAVAT WIMTDNPRTA SARDDFFRAS AMQLLTALIA DVCLSGHTET EDQTLRRVRA
     NLSEPEPKLR ARLTKIYEGS ESDFVKENVS VFVNMTPETF SGVYANAVKE THWLSYPNYA
     GLVSGDSFST DDLADGGTDI FIALDLKVLE AHPGLARVVI GSLLNAIYNR NGNVKGRTLF
     LLDEVARLGY LRILETARDA GRKYGIMLTM IFQSLGQMRE AYGGRDATSK WFESASWISF
     AAINDPDTAD YISKRCGDTT VEVDQTNRST GMKGSSRSRS KQLSRRPLIL PHEVLHMRSD
     EQIVFTSGNP PLRCGRAIWF RRDDMKACVG ENRFHRTGTG TDTHTEHAPP WQKEGTRP