ID   TRAG_SINFN              Reviewed;         640 AA.
AC   P55421;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Probable conjugal transfer protein TraG;
GN   Name=traG; OrderedLocusNames=NGR_a03950; ORFNames=y4dV;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00090; AAB92442.1; -; Genomic_DNA.
DR   RefSeq; NP_443831.1; NC_000914.2.
DR   RefSeq; WP_010875407.1; NC_000914.2.
DR   AlphaFoldDB; P55421; -.
DR   STRING; 394.NGR_a03950; -.
DR   EnsemblBacteria; AAB92442; AAB92442; NGR_a03950.
DR   KEGG; rhi:NGR_a03950; -.
DR   PATRIC; fig|394.7.peg.416; -.
DR   eggNOG; COG3505; Bacteria.
DR   HOGENOM; CLU_028732_0_0_5; -.
DR   OMA; ASWIMSD; -.
DR   OrthoDB; 246216at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014135; Ti-typ_conjug_TS_TraG-like.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02767; TraG-Ti; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Conjugation; Membrane; Plasmid; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..640
FT                   /note="Probable conjugal transfer protein TraG"
FT                   /id="PRO_0000221656"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   640 AA;  69180 MW;  12810EFE1CC8B874 CRC64;
     MTVNRIALVV APVVLMVLTV IGMSGIEQCL STFGKTDAAR LALGRIGIAF PYVTAAAIAV
     IFLFASAGSA IIKLAGWGAL AGSAATMLIA TMREASRLAA FAAQVPAGKS IASYLDHATL
     IGATAALMSG CFSLRVALIG NAAFARAEPR RIRGKRALHG EADWMNMQDA ARLFSDAGGV
     VIGERYRVDK DSTAARSFRA DDPETWGAGG KSPLLCFDGS FGSSHGIVFA GSGGFKTTSV
     TIPTALKWGG ALVVLDPSNE VAPMVYEHRK AAARFIRILD PKEPETGFNA LDWIGRFGGT
     KEEDIASVAS WIMSDSGGMR GVRDDFFRAS ALQLLTALIA DVCLSGHTDK ENQTLRQVRA
     NLAEPEPKLR ERLQSIYDNS NSDFVKENVA AYVNMTPETF SGVYANAVKE THWLSYPNYA
     ALVSGSTFST DALAAGETDV FINIDLKTLE THAGLARVII GSFLNAIYNR DGEVKGRALF
     FLDEVARLGY MRILETARDA GRKYGIMLTM IYQSIGQMRE TYGGRDAASK WFESASWISF
     AAINDPETAD YISRRCGMTT VEIDQVSRSF QTKGSSRTRS KQLAARPLIQ PHEVLRMRAD
     EQIVFTAGNA PLRCGRAIWF RREDMKACGG EPVSQGRKDV