ID   TRAI5_ECOLX             Reviewed;         747 AA.
AC   Q00192;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Protein TraI;
GN   Name=traI;
OS   Escherichia coli.
OG   Plasmid IncP-beta R751.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1665997; DOI=10.3109/10425179109020786;
RA   Ziegelin G., Pansegrau W., Strack B., Balzer D., Kroeger M., Kruft V.,
RA   Lanka E.;
RT   "Nucleotide sequence and organization of genes flanking the transfer origin
RT   of promiscuous plasmid RP4.";
RL   DNA Seq. 1:303-327(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Thomas C.M.;
RL   Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The initiation process of transfer DNA synthesis requires the
CC       interaction of at least three plasmid-specific components (TraH, TraI,
CC       and TraJ) at the transfer origin resulting in the assembly of a
CC       specialized nucleoprotein complex - the relaxosome. Site and strand
CC       specific cleavage at the transfer origin is dependent on TraI and TraJ.
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DR   EMBL; X54458; CAA38329.1; -; Genomic_DNA.
DR   EMBL; U67194; AAC64475.1; -; Genomic_DNA.
DR   PIR; S22994; S22994.
DR   AlphaFoldDB; Q00192; -.
DR   SMR; Q00192; -.
DR   InterPro; IPR005094; Endonuclease_MobA/VirD2.
DR   InterPro; IPR040677; LPD7.
DR   Pfam; PF18821; LPD7; 1.
DR   Pfam; PF03432; Relaxase; 1.
PE   4: Predicted;
KW   Conjugation; Plasmid.
FT   CHAIN           1..747
FT                   /note="Protein TraI"
FT                   /id="PRO_0000068454"
FT   REGION          510..594
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          624..652
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        510..528
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        559..585
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   747 AA;  82195 MW;  69E59666E039535D CRC64;
     MIAKHVPMRS LGKSDFAGLA NYITDAQSKD HRLGHVQATN CEAGSIQDAI TEVLATQHTN
     TRAKGDKTYH LIVSFRAGEQ PSADTLRAIE ERICVGLGYG EHQRISAVHN DTDNLHIHIA
     INKIHPTRHT MHEPYYPHRA LAELCTALER DYGLERDNHE PRKRGAEGRA ADMERHAGVE
     SLVGWIKREC LDEIKGAQSW QELHQVMRDN GMELRVRANG LVFEAGDGTM VKASTVARDL
     SKPSLEARLG PFEASPERQA QTTAKRQYRK DPIRLRVNTV ELYAKYKAEQ QSLTTARAQA
     LERARHRKDR LIEAAKRSNR LRRATIKVVG EGRANKKLLY AQASKALRSE IQAINKQFQQ
     ERTALYAEHS RRTWADWLKK EAQHGGADAL AALRAREAAQ GLKGNTIRGE GQAKPGHAPA
     VDNITKKGTI IFRAGMSAVR DDGDRLQVSR EATREGLQEA LRLAMQRYGN RITVNGTVEF
     KAQMIRAAVD SQLPITFTDP ALESRRQALL NKENTHERTE RPEHRGRTGR GAGGPGQRPA
     ADQHATGAAA VARAGDGRPA AGRGDRADAG LHAATVHRKP DVGRLGRKPP PQSQHRLRAL
     SELGVVRIAG GSEVLLPRDV PRHVEQQGTQ PDHALRRGIS RPGTGVGQTP PGVAAADKYI
     AEREAKRLKG FDIPKHSRYT AGDGALTFQG TRTIEGQALA LLKRGDEVMV MPIDQATARR
     LTRIAVGRDA VSITAKGSIK TSKGRSR