ID   TRAI6_ECOLX             Reviewed;         732 AA.
AC   Q00191;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Protein TraI;
GN   Name=traI;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-8.
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1665997; DOI=10.3109/10425179109020786;
RA   Ziegelin G., Pansegrau W., Strack B., Balzer D., Kroeger M., Kruft V.,
RA   Lanka E.;
RT   "Nucleotide sequence and organization of genes flanking the transfer origin
RT   of promiscuous plasmid RP4.";
RL   DNA Seq. 1:303-327(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-15.
RX   PubMed=2168553; DOI=10.1073/pnas.87.17.6555;
RA   Pansegrau W., Balzer D., Kruft V., Lurz R., Lanka E.;
RT   "In vitro assembly of relaxosomes at the transfer origin of plasmid RP4.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:6555-6559(1990).
CC   -!- FUNCTION: The initiation process of transfer DNA synthesis requires the
CC       interaction of at least three plasmid-specific components (TraH, I, and
CC       J) at the transfer origin resulting in the assembly of a specialised
CC       nucleoprotein complex - the relaxosome. Site- and strand-specific
CC       cleavage at the transfer origin is dependent on TraI and TraJ.
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DR   EMBL; X54459; CAA38336.1; -; Genomic_DNA.
DR   PIR; S23001; S23001.
DR   RefSeq; WP_011205818.1; NZ_NJTR01000055.1.
DR   AlphaFoldDB; Q00191; -.
DR   PRIDE; Q00191; -.
DR   InterPro; IPR005094; Endonuclease_MobA/VirD2.
DR   InterPro; IPR040677; LPD7.
DR   Pfam; PF18821; LPD7; 1.
DR   Pfam; PF03432; Relaxase; 1.
PE   1: Evidence at protein level;
KW   Conjugation; Direct protein sequencing; Plasmid.
FT   CHAIN           1..732
FT                   /note="Protein TraI"
FT                   /id="PRO_0000068455"
FT   REGION          508..553
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        508..531
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   732 AA;  81563 MW;  BD97EA7DB14E96AF CRC64;
     MIAKHVPMRS IKKSDFAELV KYITDEQGKT ERLGHVRVTN CEANTLPAVM AEVMATQHGN
     TRSEADKTYH LLVSFRAGEK PDAETLRAIE DRICAGLGFA EHQRVSAVHH DTDNLHIHIA
     INKIHPTRNT IHEPYRAYRA LADLCATLER DYGLERDNHE TRQRVSENRA NDMERHAGVE
     SLVGWIKREC LPELQAAQSW EDLHRVLREN GLKLRERGNG FIFEAGDGTT VKASTVSRDL
     SKPKLEARFG AFTPAEGGEA PRRREYRAKP LKTRIDTTEL YARYQSERQE MGAVRKGELD
     TLRRRRDRLI EAAMRSNRLR RAAIKLLGEG RIAKRLMYAQ AHKALRADLD KINREYRQGR
     QAVQERTQRR AWADWLKAEA MKGDDKALAA LRAREGRSDL KGNTIQGSGE AKPGHAAVTD
     NITKKGTIIY RVGSSAVRDD GDRLQVSREA TTDGLDAALR LAMERFGDRI TVNGTAEFKE
     RIAQAAAAGR LAITFDDAAL ERRRQELLTK EQAHEQPERN DGRRDRGGDG GIRPAAARTT
     LNATGGDGDR RDARAVSAGG TVALRKPNVG RIGRKPPPQS QNRLRALSQL GVVRIAGGAE
     MLLPRDVPGH VEQQGAEPAH ALRRGVSGPG RGLKPEQIAA AEKYVAEREQ KRLNGFDIPK
     HARYTDYVGA LSYAGTRNVE DQALALLRKE NDEILVLPVD KATVQRMKRL AIGDPVTVTP
     RGSLKTTRGR SR