ID   TRAI_AGRFC              Reviewed;         211 AA.
AC   P33907;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Acyl-homoserine-lactone synthase;
DE            EC=2.3.1.184;
DE   AltName: Full=Autoinducer synthesis protein TraI;
GN   Name=traI; OrderedLocusNames=Atu6042; ORFNames=AGR_pTi_91;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8197112; DOI=10.1073/pnas.91.11.4639;
RA   Hwang I., Li P.-L., Zhang L., Piper K.R., Cook D.M., Tate M.E.,
RA   Farrand S.K.;
RT   "TraI, a LuxI homologue, is responsible for production of conjugation
RT   factor, the Ti plasmid N-acylhomoserine lactone autoinducer.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:4639-4643(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9829924; DOI=10.1128/jb.180.23.6164-6172.1998;
RA   Li P.-L., Everhart D.M., Farrand S.K.;
RT   "Genetic and sequence analysis of the pTiC58 trb locus, encoding a mating-
RT   pair formation system related to members of the type IV secretion family.";
RL   J. Bacteriol. 180:6164-6172(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-
CC       homoserine lactone), an autoinducer molecule which binds to TraR and
CC       thus acts in the control of conjugal transfer.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-
CC         homoserine lactone + H(+) + holo-[ACP] + S-methyl-5'-thioadenosine;
CC         Xref=Rhea:RHEA:10096, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:55474,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64479, ChEBI:CHEBI:138651;
CC         EC=2.3.1.184;
CC   -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00533}.
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DR   EMBL; L22207; AAA27405.1; -; Genomic_DNA.
DR   EMBL; AF057718; AAC82627.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK91000.1; -; Genomic_DNA.
DR   PIR; AH3232; AH3232.
DR   RefSeq; NP_396559.1; NC_003065.3.
DR   RefSeq; WP_010974838.1; NC_003065.3.
DR   AlphaFoldDB; P33907; -.
DR   SMR; P33907; -.
DR   EnsemblBacteria; AAK91000; AAK91000; Atu6042.
DR   GeneID; 39477058; -.
DR   KEGG; atu:Atu6042; -.
DR   PATRIC; fig|176299.10.peg.5248; -.
DR   HOGENOM; CLU_085711_4_0_5; -.
DR   OMA; NCINGME; -.
DR   PhylomeDB; P33907; -.
DR   BioCyc; AGRO:ATU6042-MON; -.
DR   BioCyc; MetaCyc:MON-14566; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0061579; F:N-acyl homoserine lactone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR018311; Autoind_synth_CS.
DR   InterPro; IPR001690; Autoind_synthase.
DR   PANTHER; PTHR39322; PTHR39322; 1.
DR   Pfam; PF00765; Autoind_synth; 1.
DR   PRINTS; PR01549; AUTOINDCRSYN.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS00949; AUTOINDUCER_SYNTH_1; 1.
DR   PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE   3: Inferred from homology;
KW   Autoinducer synthesis; Conjugation; Plasmid; Quorum sensing;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..211
FT                   /note="Acyl-homoserine-lactone synthase"
FT                   /id="PRO_0000210898"
SQ   SEQUENCE   211 AA;  23452 MW;  497999CBDD0A0589 CRC64;
     MRILTVSPDQ YERYRSFLKQ MHRLRATVFG GRLEWDVSII AGEERDQYDN FKPSYLLAIT
     DSGRVAGCVR LLPACGPTML EQTFSQLLEM GSLAAHSGMV ESSRFCVDTS LVSRRDASQL
     HLATLTLFAG IIEWSMASGY TEIVTATDLR FERILKRAGW PMRRLGEPTA IGNTIAIAGR
     LPADRASFEQ VCPPGYYSIP RIDVAAIRSA A