ID   TRAI_RHIRD              Reviewed;         212 AA.
AC   P33904;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Acyl-homoserine-lactone synthase;
DE            EC=2.3.1.184;
DE   AltName: Full=Autoinducer synthesis protein TraI;
GN   Name=traI;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A348;
RX   PubMed=8188582; DOI=10.1128/jb.176.10.2796-2806.1994;
RA   Fuqua W.C. Jr., Winans S.C.;
RT   "A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid
RT   conjugal transfer in the presence of a plant tumor metabolite.";
RL   J. Bacteriol. 176:2796-2806(1994).
CC   -!- FUNCTION: Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-
CC       homoserine lactone), an autoinducer molecule which binds to TraR and
CC       thus acts in the control of conjugal transfer.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-
CC         homoserine lactone + H(+) + holo-[ACP] + S-methyl-5'-thioadenosine;
CC         Xref=Rhea:RHEA:10096, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:55474,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64479, ChEBI:CHEBI:138651;
CC         EC=2.3.1.184;
CC   -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00533}.
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DR   EMBL; L17024; AAA64792.1; -; Genomic_DNA.
DR   EMBL; AF242881; AAB95104.1; -; Genomic_DNA.
DR   RefSeq; NP_059761.1; NC_002377.1.
DR   RefSeq; WP_010892449.1; NC_002377.1.
DR   AlphaFoldDB; P33904; -.
DR   SMR; P33904; -.
DR   GO; GO:0061579; F:N-acyl homoserine lactone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR018311; Autoind_synth_CS.
DR   InterPro; IPR001690; Autoind_synthase.
DR   PANTHER; PTHR39322; PTHR39322; 1.
DR   Pfam; PF00765; Autoind_synth; 1.
DR   PRINTS; PR01549; AUTOINDCRSYN.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS00949; AUTOINDUCER_SYNTH_1; 1.
DR   PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE   3: Inferred from homology;
KW   Autoinducer synthesis; Conjugation; Plasmid; Quorum sensing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..212
FT                   /note="Acyl-homoserine-lactone synthase"
FT                   /id="PRO_0000210899"
SQ   SEQUENCE   212 AA;  23412 MW;  E1D6D772E2A9F7C2 CRC64;
     MLILTVSPDQ YQHQNSYLKQ MHRLRAEVFG NRLKWDVAIE DGGERDQYDE LSPTYILATF
     GGQRVVGCAR LLAPSGPTML ERTFPQLLAT GSLSATTAMI ETSRFCVDTT LPTGRAGRQL
     HLATLTMFAG IIEWSMANGY DEIVTATDLR FERILKRAGW PMTRLGEPVA IGNTVAVAGH
     LPADRKSFER VCPPGYRSII ADDNGRPLRS AA