ID   TRAJ1_ECOLI             Reviewed;         229 AA.
AC   P06626;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Protein TraJ;
GN   Name=traJ; OrderedLocusNames=ECOK12F072;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6368316; DOI=10.1016/0378-1119(83)90038-0;
RA   Fowler T., Taylor L., Thompson R.;
RT   "The control region of the F plasmid transfer operon: DNA sequence of the
RT   traJ and traY genes and characterisation of the traY leads to Z promoter.";
RL   Gene 26:79-89(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RX   PubMed=6298579; DOI=10.1007/bf00330058;
RA   Thompson R., Taylor L.;
RT   "Promoter mapping and DNA sequencing of the F plasmid transfer genes traM
RT   and traJ.";
RL   Mol. Gen. Genet. 188:513-518(1982).
CC   -!- FUNCTION: This protein is essential for positively regulating the
CC       expression of transfer genes that are involved in the conjugal transfer
CC       of DNA between bacterial cells.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; K01147; AAA24908.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44188.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97942.1; -; Genomic_DNA.
DR   EMBL; X00545; CAA25217.1; -; Genomic_DNA.
DR   PIR; A21874; BVECTJ.
DR   RefSeq; NP_061451.1; NC_002483.1.
DR   RefSeq; NP_862917.1; NC_004998.1.
DR   RefSeq; WP_000283385.1; NZ_SSUW01000046.1.
DR   RefSeq; YP_003937615.1; NC_014615.1.
DR   RefSeq; YP_009060158.1; NC_024956.1.
DR   RefSeq; YP_009068353.1; NC_025139.1.
DR   RefSeq; YP_009070618.1; NC_025175.1.
DR   RefSeq; YP_009071228.1; NC_025179.1.
DR   PDB; 4KQD; X-ray; 1.55 A; A/B/C/D=14-133.
DR   PDBsum; 4KQD; -.
DR   AlphaFoldDB; P06626; -.
DR   SMR; P06626; -.
DR   IntAct; P06626; 1.
DR   PRIDE; P06626; -.
DR   PATRIC; fig|83333.107.peg.641; -.
DR   PRO; PR:P06626; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR016383; TraJ.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PIRSF; PIRSF003267; TraJ_F; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Conjugation; Cytoplasm; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..229
FT                   /note="Protein TraJ"
FT                   /id="PRO_0000068456"
FT   HELIX           17..25
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   STRAND          32..36
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   STRAND          41..44
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   HELIX           46..52
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   HELIX           59..65
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   HELIX           70..80
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   STRAND          88..96
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   STRAND          99..110
FT                   /evidence="ECO:0007829|PDB:4KQD"
FT   STRAND          113..127
FT                   /evidence="ECO:0007829|PDB:4KQD"
SQ   SEQUENCE   229 AA;  27062 MW;  DBCD92A0465A0DA9 CRC64;
     MYPMDRIQQK HARQIDLLEN LTAVIQDYPN PACIRDETGK FIFCNTLFHE SFLTQDQSAE
     KWLLSQRDFC ELISVTEMEA YRNEHTHLNL VEDVFIQNRF WTISVQSFLN GHRNIILWQF
     YDAAHVRHKD SYNQKTIVSD DIRNIIRRMS DDSSVSSYVN DVFYLYSTGI SHNAIARILN
     ISISTSKKHA SLICDYFSVS NKDELIILLY NKKFIYYLYE KAMCIINTR