ID   TRAJ2_ECOLX             Reviewed;         228 AA.
AC   P05837;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 3.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Protein TraJ;
GN   Name=traJ;
OS   Escherichia coli.
OG   Plasmid IncFII R1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3012278; DOI=10.1007/bf00330394;
RA   Koronakis V., Hoegenauer G.;
RT   "The sequences of the traJ gene and the 5' end of the traY gene of the
RT   resistance plasmid R1.";
RL   Mol. Gen. Genet. 203:137-142(1986).
RN   [2]
RP   SEQUENCE REVISION TO 41.
RA   Koraimann G.M.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-228.
RX   PubMed=2564189; DOI=10.1093/nar/17.4.1283;
RA   Koraimann G.M., Hoegenauer G.;
RT   "A stable core region of the tra operon mRNA of plasmid R1-19.";
RL   Nucleic Acids Res. 17:1283-1298(1989).
RN   [4]
RP   POST-TRANSCRIPTIONAL CONTROL BY FINOP.
RX   PubMed=8878043; DOI=10.1046/j.1365-2958.1996.361401.x;
RA   Koraimann G., Teferle K., Markolin G., Woger W., Hogenauer G.;
RT   "The FinOP repressor system of plasmid R1: analysis of the antisense RNA
RT   control of traJ expression and conjugative DNA transfer.";
RL   Mol. Microbiol. 21:811-821(1996).
RN   [5]
RP   COACTIVATION OF TRANSFER GENES WITH ARCA.
RX   PubMed=9514749; DOI=10.1006/jmbi.1997.1598;
RA   Strohmaier H., Noiges R., Kotschan S., Sawers G., Hogenauer G.,
RA   Zechner E.L., Koraimann G.;
RT   "Signal transduction and bacterial conjugation: characterization of the
RT   role of ArcA in regulating conjugative transfer of the resistance plasmid
RT   R1.";
RL   J. Mol. Biol. 277:309-316(1998).
RN   [6]
RP   FUNCTION IN ACTIVATION OF TRANSFER GENES, INTERACTION WITH GROEL, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=17586648; DOI=10.1128/jb.00005-07;
RA   Zahrl D., Wagner A., Tscherner M., Koraimann G.;
RT   "GroEL plays a central role in stress-induced negative regulation of
RT   bacterial conjugation by promoting proteolytic degradation of the activator
RT   protein TraJ.";
RL   J. Bacteriol. 189:5885-5894(2007).
CC   -!- FUNCTION: Together with ArcA, is essential for positively regulating
CC       the expression of transfer genes that are involved in the conjugal
CC       transfer of DNA between bacterial cells. {ECO:0000269|PubMed:17586648}.
CC   -!- SUBUNIT: Interacts with GroEL. {ECO:0000269|PubMed:17586648}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17586648}.
CC   -!- INDUCTION: Post-transcriptionally repressed by the FinOP system.
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DR   EMBL; X13681; CAA31971.1; -; Genomic_DNA.
DR   PIR; A26021; BVECRJ.
DR   RefSeq; WP_000332487.1; NZ_WWEV01000072.1.
DR   AlphaFoldDB; P05837; -.
DR   SMR; P05837; -.
DR   OMA; QLTIWND; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013767; PAS_fold.
DR   InterPro; IPR016384; TraJ_R1.
DR   Pfam; PF00989; PAS; 1.
DR   PIRSF; PIRSF003268; TraJ_R1; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
PE   1: Evidence at protein level;
KW   Activator; Conjugation; Cytoplasm; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..228
FT                   /note="Protein TraJ"
FT                   /id="PRO_0000068457"
FT   CONFLICT        41
FT                   /note="R -> W (in Ref. 1; CAA31971)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   228 AA;  26433 MW;  3B92DE2E7DC27505 CRC64;
     MCALDRRERP LNSQSVNKYI LNVQNIYRNS PVPVCVRNKN RKILYANGAF IELFSREDKP
     LSGESYIRLQ VEIFLSSLEL ECQALGHGSA FCRRFNFHGE IYQIRMENVS FYNDESVVLW
     QINPFPDYPF FALNQSGSNT NTSDKLTIWN DLSPGTLVVF SFYMLGVGHA TIARELGITD
     RASEDRIKPV KRKIKEFFEH FDLFRVSCIY KGEIDSLLSI IREFYGVK