ID   TRAJ6_ECOLX             Reviewed;         123 AA.
AC   P17909;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Protein TraJ;
DE   AltName: Full=Relaxosome protein;
GN   Name=traJ;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-6 AND 119-123.
RX   PubMed=2663846; DOI=10.1016/s0021-9258(18)80164-8;
RA   Ziegelin G., Fuerste J.P., Lanka E.;
RT   "TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence
RT   repetition within the transfer origin.";
RL   J. Biol. Chem. 264:11989-11994(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33694 / HB101;
RX   PubMed=1665997; DOI=10.3109/10425179109020786;
RA   Ziegelin G., Pansegrau W., Strack B., Balzer D., Kroeger M., Kruft V.,
RA   Lanka E.;
RT   "Nucleotide sequence and organization of genes flanking the transfer origin
RT   of promiscuous plasmid RP4.";
RL   DNA Seq. 1:303-327(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RX   PubMed=2538813; DOI=10.1073/pnas.86.6.1771;
RA   Fuerste J.P., Pansegrau W., Ziegelin G., Kroeger M., Lanka E.;
RT   "Conjugative transfer of promiscuous IncP plasmids: interaction of plasmid-
RT   encoded products with the transfer origin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:1771-1775(1989).
RN   [4]
RP   PROTEIN SEQUENCE OF 115-123.
RX   PubMed=2168553; DOI=10.1073/pnas.87.17.6555;
RA   Pansegrau W., Balzer D., Kruft V., Lurz R., Lanka E.;
RT   "In vitro assembly of relaxosomes at the transfer origin of plasmid RP4.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:6555-6559(1990).
CC   -!- FUNCTION: Transfer of plasmid RP4 during bacterial conjugation requires
CC       the plasmid-encoded TraJ protein, which binds to a 19-base pair invert
CC       sequence repetition within the transfer origin. TraJ protein is bound
CC       to only one side of the DNA helix. This nucleoprotein structure is the
CC       initial complex in the pathway to assemble a functional relaxosome.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; J04942; AAA91588.1; -; Genomic_DNA.
DR   EMBL; X54459; CAA38338.1; -; Genomic_DNA.
DR   EMBL; M25423; AAA26421.2; -; Genomic_DNA.
DR   PIR; A34172; A34172.
DR   RefSeq; WP_008832110.1; NZ_NJTR01000055.1.
DR   AlphaFoldDB; P17909; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Conjugation; Cytoplasm; Direct protein sequencing; DNA-binding; Plasmid.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2663846"
FT   CHAIN           2..123
FT                   /note="Protein TraJ"
FT                   /id="PRO_0000068461"
SQ   SEQUENCE   123 AA;  13464 MW;  FF013C400425C6A6 CRC64;
     MADETKPTRK GSPPIKVYCL PDERRAIEEK AAAAGMSLSA YLLAVGQGYK ITGVVDYEHV
     RELARINGDL GRLGGLLKLW LTDDPRTARF GDATILALLA KIEEKQDELG KVMMGVVRPR
     AEP