ID   BUB3_DICDI              Reviewed;         331 AA.
AC   Q54DM1;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Mitotic checkpoint protein bub3;
GN   Name=bub3; ORFNames=DDB_G0292134;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Involved in cell cycle checkpoint enforcement. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WD repeat BUB3 family. {ECO:0000305}.
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DR   EMBL; AAFI02000187; EAL61423.1; -; Genomic_DNA.
DR   RefSeq; XP_629851.1; XM_629849.1.
DR   AlphaFoldDB; Q54DM1; -.
DR   SMR; Q54DM1; -.
DR   STRING; 44689.DDB0233126; -.
DR   PaxDb; Q54DM1; -.
DR   EnsemblProtists; EAL61423; EAL61423; DDB_G0292134.
DR   GeneID; 8628531; -.
DR   KEGG; ddi:DDB_G0292134; -.
DR   dictyBase; DDB_G0292134; bub3.
DR   eggNOG; KOG1036; Eukaryota.
DR   HOGENOM; CLU_038526_0_0_1; -.
DR   InParanoid; Q54DM1; -.
DR   OMA; WDPFNRK; -.
DR   PhylomeDB; Q54DM1; -.
DR   Reactome; R-DDI-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR   Reactome; R-DDI-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR   Reactome; R-DDI-176409; APC/C:Cdc20 mediated degradation of mitotic proteins.
DR   Reactome; R-DDI-179409; APC-Cdc20 mediated degradation of Nek2A.
DR   PRO; PR:Q54DM1; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:1990298; C:bub1-bub3 complex; IBA:GO_Central.
DR   GO; GO:0000776; C:kinetochore; ISS:dictyBase.
DR   GO; GO:0033597; C:mitotic checkpoint complex; IBA:GO_Central.
DR   GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 2.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome; Repeat;
KW   WD repeat.
FT   CHAIN           1..331
FT                   /note="Mitotic checkpoint protein bub3"
FT                   /id="PRO_0000327588"
FT   REPEAT          15..54
FT                   /note="WD 1"
FT   REPEAT          56..94
FT                   /note="WD 2"
FT   REPEAT          96..135
FT                   /note="WD 3"
FT   REPEAT          138..177
FT                   /note="WD 4"
FT   REPEAT          237..276
FT                   /note="WD 5"
FT   REPEAT          279..327
FT                   /note="WD 6"
SQ   SEQUENCE   331 AA;  37420 MW;  9E9C2A2945F9EF6C CRC64;
     MSNEPTSSFY ELRLPPSDGI SSVNFCPNSV NLLVTSWDST VRCYDTQNNV QKWQYNHKGP
     VMDGCFPEKN KVFSGDVFGS VKHYDPVAGV EKEVGSHEDG VRSVVYNSDT QQLFTGGWDQ
     QLKLWDIRSD KMEISNHDLQ SKIFTMDVSP ISNMLVIGTA DKYVTIYDTR QMETHLQKRE
     SSIKYQTRCI RTFTDGKGYA LASVEGRIAM EYFDPSPAVQ SKKYAFKCHR LTESGVDVVY
     PVNCIAFNPH YGTFATGGCD KNVFFWDGAN RKRLHALKTY PTSISSMSFN TDGNILAVAS
     SYTFEEGEKD HPPDQIFIHN ILSEKIIKPI K