ID   TRAM2_ECOLX             Reviewed;         127 AA.
AC   P07294;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Relaxosome protein TraM;
GN   Name=traM;
OS   Escherichia coli.
OG   Plasmid IncFII R1, and Plasmid IncFII R1-19 (R1 drd-19).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFII R1;
RX   PubMed=2998939; DOI=10.1016/0378-1119(85)90071-x;
RA   Koronakis V.E., Bauer E., Hogenauer G.;
RT   "The traM gene of the resistance plasmid R1: comparison with the
RT   corresponding sequence of the Escherichia coli F factor.";
RL   Gene 36:79-86(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFII R1-19 (R1 drd-19);
RX   PubMed=3009392; DOI=10.1128/jb.166.2.368-374.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Nucleotide sequences of the R1-19 plasmid transfer genes traM, finP, traJ,
RT   and traY and the traYZ promoter.";
RL   J. Bacteriol. 166:368-374(1986).
RN   [3]
RP   DNA-BINDING.
RC   PLASMID=IncFII R1;
RX   PubMed=2041477; DOI=10.1111/j.1365-2958.1991.tb02127.x;
RA   Schwab M., Gruber H., Hogenauer G.;
RT   "The TraM protein of plasmid R1 is a DNA-binding protein.";
RL   Mol. Microbiol. 5:439-446(1991).
RN   [4]
RP   STRUCTURE BY NMR OF 2-56.
RC   PLASMID=IncFII R1;
RX   PubMed=11258958; DOI=10.1021/bi002031c;
RA   Stockner T., Plugariu C., Koraimann G., Hogenauer G., Bermel W.,
RA   Prytulla S., Sterk H.;
RT   "Solution structure of the DNA-binding domain of TraM.";
RL   Biochemistry 40:3370-3377(2001).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Probably autoregulates its own expression.
CC       Plasmid specificity is conferred by the TraD-TraM pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC       although they do not contact each other; cooperativity is achieved by
CC       DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC       of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC       the F plasmid origin of transfer (oriT) in a site- and sequence-
CC       specific manner. Interacts with TraD. Also interacts with TraY (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR   EMBL; M19710; AAA92656.1; -; Genomic_DNA.
DR   PIR; A23956; JCECMR.
DR   RefSeq; WP_001151564.1; NZ_WWEV01000072.1.
DR   RefSeq; YP_003108299.1; NC_013122.1.
DR   RefSeq; YP_003829131.1; NC_014384.1.
DR   RefSeq; YP_003829256.1; NC_014385.1.
DR   PDB; 1DP3; NMR; -; A=2-56.
DR   PDBsum; 1DP3; -.
DR   AlphaFoldDB; P07294; -.
DR   BMRB; P07294; -.
DR   SMR; P07294; -.
DR   DIP; DIP-27656N; -.
DR   OMA; NYHEMIS; -.
DR   EvolutionaryTrace; P07294; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd14804; Tra_M; 1.
DR   Gene3D; 1.10.10.450; -; 1.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR042073; TraM_DNA-bd.
DR   InterPro; IPR007925; TRelaxosome_TraM.
DR   Pfam; PF05261; Tra_M; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Relaxosome protein TraM"
FT                   /id="PRO_0000068468"
FT   HELIX           12..26
FT                   /evidence="ECO:0007829|PDB:1DP3"
FT   HELIX           35..44
FT                   /evidence="ECO:0007829|PDB:1DP3"
FT   TURN            45..47
FT                   /evidence="ECO:0007829|PDB:1DP3"
FT   HELIX           49..52
FT                   /evidence="ECO:0007829|PDB:1DP3"
SQ   SEQUENCE   127 AA;  14446 MW;  A1490B7A3CFC2082 CRC64;
     MAKVQAYVSD EIVYKINKIV ERRRAEGAKS TDVSFSSIST MLLELGLRVY EAQMERKESA
     FNQAEFNKVL LECAVKTQST VAKILGIESL SPHVSGNPKF EYANMVEDIR DKVSSEMERF
     FPENDEE