TRAM3_ECOLX
ID TRAM3_ECOLX Reviewed; 127 AA.
AC P13973; Q46710; Q9WTE3;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Relaxosome protein TraM;
GN Name=traM;
OS Escherichia coli.
OG Plasmid IncFII R100 (NR1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RX PubMed=3522549; DOI=10.1128/jb.167.1.336-345.1986;
RA Fee B.E., Dempsey W.B.;
RT "Cloning, mapping, and sequencing of plasmid R100 traM and finP genes.";
RL J. Bacteriol. 167:336-345(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=IncFII R100 (NR1);
RA Sampei G., Mizobuchi K.;
RT "Organization and diversification of plasmid genomes: complete nucleotide
RT sequence of the R100 genome.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-127.
RC PLASMID=IncFII R100 (NR1);
RX PubMed=2836369; DOI=10.1128/jb.170.6.2749-2757.1988;
RA Inamoto S., Yoshioka Y., Ohtsubo E.;
RT "Identification and characterization of the products from the traJ and traY
RT genes of plasmid R100.";
RL J. Bacteriol. 170:2749-2757(1988).
RN [4]
RP FUNCTION IN CONFERRING PLASMID SPECIFICITY, AND MUTAGENESIS OF
RP 3-ARG--ILE-5.
RC PLASMID=IncFII R100 (NR1);
RX PubMed=21565799; DOI=10.1093/nar/gkr296;
RA Wong J.J., Lu J., Edwards R.A., Frost L.S., Glover J.N.;
RT "Structural basis of cooperative DNA recognition by the plasmid conjugation
RT factor, TraM.";
RL Nucleic Acids Res. 39:6775-6788(2011).
CC -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC of ssDNA plasmid from a donor to a recipient cell. It is the central
CC mechanism by which antibiotic resistance and virulence factors are
CC propagated in bacterial populations. Part of the relaxosome, which
CC facilitates a site- and strand-specific cut in the origin of transfer
CC by TraI, at the nic site. Probably autoregulates its own expression (By
CC similarity). Plasmid specificity is conferred by the TraD-TraM pair.
CC {ECO:0000250, ECO:0000269|PubMed:21565799}.
CC -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC although they do not contact each other; cooperativity is achieved by
CC DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC the F plasmid origin of transfer (oriT) in a site- and sequence-
CC specific manner. Interacts with TraD. Also interacts with TraY (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR EMBL; M13054; AAA88501.1; -; Genomic_DNA.
DR EMBL; AP000342; BAA78848.1; -; Genomic_DNA.
DR EMBL; M20941; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A32014; A32014.
DR RefSeq; NP_957597.1; NC_005327.1.
DR RefSeq; WP_001354030.1; NZ_WVVN01000094.1.
DR RefSeq; YP_001096465.1; NC_009133.1.
DR RefSeq; YP_006952235.1; NC_019057.1.
DR RefSeq; YP_006953319.1; NC_019071.1.
DR RefSeq; YP_006953418.1; NC_019072.1.
DR RefSeq; YP_006953945.1; NC_019090.1.
DR RefSeq; YP_006954266.1; NC_019095.1.
DR RefSeq; YP_006990757.1; NC_019424.1.
DR RefSeq; YP_007447543.1; NC_020278.2.
DR RefSeq; YP_009070964.1; NC_025177.1.
DR RefSeq; YP_788059.1; NC_008460.1.
DR AlphaFoldDB; P13973; -.
DR SMR; P13973; -.
DR GeneID; 58462405; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR CDD; cd14804; Tra_M; 1.
DR Gene3D; 1.10.10.450; -; 1.
DR InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR InterPro; IPR042073; TraM_DNA-bd.
DR InterPro; IPR007925; TRelaxosome_TraM.
DR Pfam; PF05261; Tra_M; 1.
DR SUPFAM; SSF47729; SSF47729; 1.
PE 1: Evidence at protein level;
KW Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW Transcription regulation.
FT CHAIN 1..127
FT /note="Relaxosome protein TraM"
FT /id="PRO_0000068469"
FT MUTAGEN 3..5
FT /note="RVI->KVN: 60,000-fold increase in conjugation
FT efficiency of F plasmid, no detectable conjugation of
FT plasmid pED208."
FT /evidence="ECO:0000269|PubMed:21565799"
SQ SEQUENCE 127 AA; 14508 MW; A0E48A6E6C471B83 CRC64;
MARVILYISN DVYDKVNAIV EQRRQEGARD KDISVSGTAS MLLELGLRVY EAQMERKESA
FNQTEFNKLL LECVVKTQSS VAKILGIESL SPHVSGNPKF EYANMVEDIR EKVSSEMERF
FPKNDEE
