ID   TRAM6_ECOLX             Reviewed;         127 AA.
AC   P18808;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Relaxosome protein TraM;
GN   Name=traM;
OS   Escherichia coli.
OG   Plasmid IncFI P307.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=711;
RX   PubMed=2096398; DOI=10.1016/0147-619x(90)90014-4;
RA   Graus-Goeldner A., Graus H., Schlacher T., Hoegenauer G.;
RT   "The sequences of genes bordering oriT in the enterotoxin plasmid P307:
RT   comparison with the sequences of plasmids F and R1.";
RL   Plasmid 24:119-131(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
RX   PubMed=2827206; DOI=10.1016/0147-619x(87)90080-1;
RA   Goeldner A., Graus H., Hoegenauer G.;
RT   "The origin of transfer of P307.";
RL   Plasmid 18:76-83(1987).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Probably autoregulates its own expression.
CC       Plasmid specificity is conferred by the TraD-TraM pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC       although they do not contact each other; cooperativity is achieved by
CC       DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC       of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC       the F plasmid origin of transfer (oriT) in a site- and sequence-
CC       specific manner. Interacts with TraD. Also interacts with TraY (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR   EMBL; M62986; AAA25522.1; -; Genomic_DNA.
DR   EMBL; X06534; CAA29783.1; -; Genomic_DNA.
DR   PIR; B37390; B37390.
DR   RefSeq; WP_001151534.1; NZ_NTNJ01000036.1.
DR   AlphaFoldDB; P18808; -.
DR   SMR; P18808; -.
DR   PATRIC; fig|562.6988.peg.1142; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd14804; Tra_M; 1.
DR   Gene3D; 1.10.10.450; -; 1.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR042073; TraM_DNA-bd.
DR   InterPro; IPR007925; TRelaxosome_TraM.
DR   Pfam; PF05261; Tra_M; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
PE   3: Inferred from homology;
KW   Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Relaxosome protein TraM"
FT                   /id="PRO_0000068470"
SQ   SEQUENCE   127 AA;  14556 MW;  1F67755E2F394C99 CRC64;
     MAKVNLYISN DAYEKINAII EKRRQEGARE KDVSFSATAS MLLELGLRVY EAQMERKESA
     FNQAEFNKLL LECVVKTQST VAKILGIESL SPHVSGNPKF EYSNMVEDIR EKVSVEMERF
     FPKNDDE