ID   TRAM7_ECOLX             Reviewed;         127 AA.
AC   P33787;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Relaxosome protein TraM;
GN   Name=traM;
OS   Escherichia coli.
OG   Plasmid IncFVII pSU233.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1406590; DOI=10.1007/bf00538704;
RA   Salazar L., Lopez J., Andres I., Ortiz J.M., Rodriguez J.C.;
RT   "Characterization and nucleotide sequence of the oriT-traM-finP region of
RT   the IncFVII plasmid pSU233.";
RL   Mol. Gen. Genet. 234:442-448(1992).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Probably autoregulates its own expression.
CC       Plasmid specificity is conferred by the TraD-TraM pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC       although they do not contact each other; cooperativity is achieved by
CC       DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC       of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC       the F plasmid origin of transfer (oriT) in a site- and sequence-
CC       specific manner. Interacts with TraD. Also interacts with TraY (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR   EMBL; X55896; CAA39383.1; -; Genomic_DNA.
DR   PIR; S26249; S26249.
DR   RefSeq; WP_001151530.1; NZ_WVVL01000081.1.
DR   RefSeq; YP_008997961.1; NC_023315.1.
DR   AlphaFoldDB; P33787; -.
DR   SMR; P33787; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd14804; Tra_M; 1.
DR   Gene3D; 1.10.10.450; -; 1.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR042073; TraM_DNA-bd.
DR   InterPro; IPR007925; TRelaxosome_TraM.
DR   Pfam; PF05261; Tra_M; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
PE   3: Inferred from homology;
KW   Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Relaxosome protein TraM"
FT                   /id="PRO_0000068471"
SQ   SEQUENCE   127 AA;  14526 MW;  FC46420E2F394C9F CRC64;
     MAKVNLYISN DAYEKINAII EKRRQEGARE KDVSFSATAS MLLELGLRVY EAQMERKESA
     FNQAEFNKLL LECVVKTQSS VAKILGIESL SPHVSGNPKF EYANMVEDIR EKVSVEMERF
     FPKNDDE