ID   TRAM8_ECOLX             Reviewed;         127 AA.
AC   P33788;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Relaxosome protein TraM;
GN   Name=traM;
OS   Escherichia coli.
OG   Plasmid IncFV pED208.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-5.
RC   PLASMID=IncFV pED208;
RX   PubMed=1943709; DOI=10.1111/j.1365-2958.1991.tb01927.x;
RA   di Laurenzio L., Frost L.S., Finlay B.B., Paranchych W.;
RT   "Characterization of the oriT region of the IncFV plasmid pED208.";
RL   Mol. Microbiol. 5:1779-1790(1991).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 1-52, X-RAY CRYSTALLOGRAPHY (2.87
RP   ANGSTROMS) IN COMPLEX WITH DNA, DNA-BINDING, AND SUBUNIT.
RC   PLASMID=IncFV pED208;
RX   PubMed=21565799; DOI=10.1093/nar/gkr296;
RA   Wong J.J., Lu J., Edwards R.A., Frost L.S., Glover J.N.;
RT   "Structural basis of cooperative DNA recognition by the plasmid conjugation
RT   factor, TraM.";
RL   Nucleic Acids Res. 39:6775-6788(2011).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site (By similarity). TraM binds to three principal
CC       regions in the oriT (transfer origin) region; 2 are required for
CC       autoregulation while the other is required for plasmid transfer.
CC       Plasmid specificity is conferred by the TraD-TraM pair. {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC       although they do not contact each other; cooperativity is achieved by
CC       DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC       of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC       the F plasmid origin of transfer (oriT) in a site- and sequence-
CC       specific manner. Interacts with TraD. Probably also interacts with
CC       TraY. {ECO:0000269|PubMed:21565799}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=To a smaller extent in the inner
CC       membrane by association with another protein, probably TraD.
CC   -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR   EMBL; X59611; CAA42174.1; -; Genomic_DNA.
DR   PIR; S16476; S16476.
DR   RefSeq; WP_015062650.1; NZ_CP047278.1.
DR   PDB; 3OMY; X-ray; 1.30 A; A/B=1-52.
DR   PDB; 3ON0; X-ray; 2.87 A; A/B/C/D=1-127.
DR   PDBsum; 3OMY; -.
DR   PDBsum; 3ON0; -.
DR   AlphaFoldDB; P33788; -.
DR   SMR; P33788; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd14804; Tra_M; 1.
DR   Gene3D; 1.10.10.450; -; 1.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR042073; TraM_DNA-bd.
DR   InterPro; IPR007925; TRelaxosome_TraM.
DR   Pfam; PF05261; Tra_M; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Conjugation; Cytoplasm; Direct protein sequencing;
KW   DNA-binding; Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Relaxosome protein TraM"
FT                   /id="PRO_0000068472"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:3ON0"
FT   HELIX           10..25
FT                   /evidence="ECO:0007829|PDB:3OMY"
FT   HELIX           33..51
FT                   /evidence="ECO:0007829|PDB:3OMY"
FT   STRAND          55..58
FT                   /evidence="ECO:0007829|PDB:3ON0"
FT   HELIX           61..88
FT                   /evidence="ECO:0007829|PDB:3ON0"
FT   HELIX           90..95
FT                   /evidence="ECO:0007829|PDB:3ON0"
FT   HELIX           100..118
FT                   /evidence="ECO:0007829|PDB:3ON0"
FT   HELIX           121..123
FT                   /evidence="ECO:0007829|PDB:3ON0"
SQ   SEQUENCE   127 AA;  14665 MW;  C0BEDB1D0A71FE07 CRC64;
     MPKIQTYVNN NVYEQITDLV TIRKQEGIEE ASLSNVSSML LELGLRVYMI QQEKREGGFN
     QMEYNKLMLE NVSRVRAMCT EILKMSVLNQ ESIASGNFDY AVIKPAIDKF AREQVSIFFP
     DDEDDQE