ID   TRAM9_ECOLX             Reviewed;         127 AA.
AC   P18807;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Relaxosome protein TraM;
GN   Name=traM;
OS   Escherichia coli.
OG   Plasmid ColB4-K98.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3531163; DOI=10.1128/jb.168.1.132-139.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Origin of transfer of IncF plasmids and nucleotide sequences of the type
RT   II oriT, traM, and traY alleles from ColB4-K98 and the type IV traY allele
RT   from R100-1.";
RL   J. Bacteriol. 168:132-139(1986).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Probably autoregulates its own expression.
CC       Plasmid specificity is conferred by the TraD-TraM pair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. 2 homotetramers cooperatively bind to DNA
CC       although they do not contact each other; cooperativity is achieved by
CC       DNA kinking and unwinding. Part of the relaxosome, a complex composed
CC       of plasmid encoded TraI, TraM, TraY and host-encoded IHF which binds to
CC       the F plasmid origin of transfer (oriT) in a site- and sequence-
CC       specific manner. Interacts with TraD. Also interacts with TraY (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the relaxosome TraM family. {ECO:0000305}.
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DR   EMBL; M15134; AAB04664.1; -; Genomic_DNA.
DR   PIR; A25033; JCECMK.
DR   RefSeq; WP_000124981.1; NZ_WVVZ01000053.1.
DR   RefSeq; YP_001965451.1; NC_010862.1.
DR   RefSeq; YP_006953517.1; NC_019073.1.
DR   RefSeq; YP_006953838.1; NC_019089.1.
DR   RefSeq; YP_009066475.1; NC_025106.1.
DR   AlphaFoldDB; P18807; -.
DR   SMR; P18807; -.
DR   OMA; NCIDEIH; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd14804; Tra_M; 1.
DR   Gene3D; 1.10.10.450; -; 1.
DR   InterPro; IPR010992; IHF-like_DNA-bd_dom_sf.
DR   InterPro; IPR042073; TraM_DNA-bd.
DR   InterPro; IPR007925; TRelaxosome_TraM.
DR   Pfam; PF05261; Tra_M; 1.
DR   SUPFAM; SSF47729; SSF47729; 1.
PE   3: Inferred from homology;
KW   Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..127
FT                   /note="Relaxosome protein TraM"
FT                   /id="PRO_0000068473"
SQ   SEQUENCE   127 AA;  14543 MW;  58CB49A31C84569C CRC64;
     MARVNLYISN EVHEKINMIV EKRRQEGARD KDISLSGTAS MLLELGLRVY DAQMERKESA
     FNQTEFNKLL LECVVKTQST VAKILGIESL SPHVSGNPKF EYASMVDDIR EKVSVEMDRF
     FPKNDDE