BUB3_SCHPO
ID BUB3_SCHPO Reviewed; 320 AA.
AC O42860;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Mitotic checkpoint protein bub3;
GN Name=bub3; ORFNames=SPAC23H3.08c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP INTERACTION WITH MAD3.
RX PubMed=11909965; DOI=10.1128/mcb.22.8.2728-2742.2002;
RA Millband D.N., Hardwick K.G.;
RT "Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-
RT promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and
RT Mph1p-dependent manner.";
RL Mol. Cell. Biol. 22:2728-2742(2002).
RN [3]
RP FUNCTION, INTERACTION WITH BUB1 AND MAD3, PHOSPHORYLATION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15509783; DOI=10.1128/mcb.24.22.9786-9801.2004;
RA Vanoosthuyse V., Valsdottir R., Javerzat J.-P., Hardwick K.G.;
RT "Kinetochore targeting of fission yeast Mad and Bub proteins is essential
RT for spindle checkpoint function but not for all chromosome segregation
RT roles of Bub1p.";
RL Mol. Cell. Biol. 24:9786-9801(2004).
CC -!- FUNCTION: Involved in cell cycle checkpoint enforcement. Involved in
CC recruitment of checkpoint proteins bub1 and mad3 to the kinetochores,
CC ensuring correct spindle checkpoint function.
CC {ECO:0000269|PubMed:15509783}.
CC -!- SUBUNIT: Interacts with bub1 and mad3. {ECO:0000269|PubMed:11909965,
CC ECO:0000269|PubMed:15509783}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15509783}.
CC Chromosome, centromere, kinetochore {ECO:0000269|PubMed:15509783}.
CC Chromosome, centromere {ECO:0000269|PubMed:15509783}. Note=Associates
CC with kinetochores and centromeres during the early stages of mitosis.
CC -!- PTM: Phosphorylated by bub1. {ECO:0000269|PubMed:15509783}.
CC -!- SIMILARITY: Belongs to the WD repeat BUB3 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB16243.1; -; Genomic_DNA.
DR PIR; T38301; T38301.
DR RefSeq; NP_593798.1; NM_001019227.2.
DR AlphaFoldDB; O42860; -.
DR SMR; O42860; -.
DR BioGRID; 278347; 59.
DR DIP; DIP-39398N; -.
DR IntAct; O42860; 3.
DR STRING; 4896.SPAC23H3.08c.1; -.
DR iPTMnet; O42860; -.
DR MaxQB; O42860; -.
DR PaxDb; O42860; -.
DR PRIDE; O42860; -.
DR EnsemblFungi; SPAC23H3.08c.1; SPAC23H3.08c.1:pep; SPAC23H3.08c.
DR GeneID; 2541856; -.
DR KEGG; spo:SPAC23H3.08c; -.
DR PomBase; SPAC23H3.08c; bub3.
DR VEuPathDB; FungiDB:SPAC23H3.08c; -.
DR eggNOG; KOG1036; Eukaryota.
DR HOGENOM; CLU_038526_0_0_1; -.
DR InParanoid; O42860; -.
DR OMA; WDPFNRK; -.
DR PhylomeDB; O42860; -.
DR Reactome; R-SPO-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR PRO; PR:O42860; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:1990298; C:bub1-bub3 complex; IMP:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0033597; C:mitotic checkpoint complex; IBA:GO_Central.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1990942; P:mitotic metaphase chromosome recapture; IMP:PomBase.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; IMP:PomBase.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IBA:GO_Central.
DR GO; GO:0140499; P:negative regulation of mitotic spindle assembly checkpoint signaling; IMP:PomBase.
DR GO; GO:0032091; P:negative regulation of protein binding; IMP:PomBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Kinetochore; Mitosis;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..320
FT /note="Mitotic checkpoint protein bub3"
FT /id="PRO_0000234560"
FT REPEAT 10..50
FT /note="WD 1"
FT REPEAT 53..91
FT /note="WD 2"
FT REPEAT 93..132
FT /note="WD 3"
FT REPEAT 231..270
FT /note="WD 4"
SQ SEQUENCE 320 AA; 35804 MW; A5028C58CEBEED43 CRC64;
MNFSKTLLKN SKDGISSVIF SPSVKNELIA GCWDGSLLHY QISENPELLG KYDLSSPILS
LEYTDEKTAL VGNLDGTVTT LDLNTRNHEF LGNHGKGVSC ISKLRLENCF ISGSWDKSFR
VWDVRVKQPV EGQDIGKKIF ASSSRDNILV LGCSERENLV YDIRNLKLPF QRRPSSFKYM
TRSVCCNQNF EGFVSSSIEG RTSVEYINPS QEAQSKNFTF KCHRQIQKDY DIVYPVNDLK
FHPIHQTLAT AGGDGVVAFW DIQVRKRLRV LNPSKINISS ISFNVDGSML AIATCAQEEA
AGNIYVHALE SNFAAPKLKS
