BUB3_XENLA
ID BUB3_XENLA Reviewed; 330 AA.
AC Q9YGY3; Q98UH2;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Mitotic checkpoint protein BUB3;
DE Short=xbub3;
DE AltName: Full=WD repeat protein Bub3;
GN Name=bub3;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo, and Oocyte;
RX PubMed=10098604; DOI=10.1089/104454999315448;
RA Goto T., Kinoshita T.;
RT "Maternal transcripts of mitotic checkpoint gene, Xbub3, are accumulated in
RT the animal blastomeres of Xenopus early embryo.";
RL DNA Cell Biol. 18:227-234(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INTERACTION WITH BUB1.
RC TISSUE=Oocyte;
RX PubMed=11231148; DOI=10.1016/s0960-9822(01)00045-8;
RA Schwab M.S., Roberts B.T., Gross S.D., Tunquist B.J., Taieb F.E.,
RA Lewellyn A.L., Maller J.L.;
RT "Bub1 is activated by the protein kinase p90(Rsk) during Xenopus oocyte
RT maturation.";
RL Curr. Biol. 11:141-150(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Oocyte;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION, AND COMPLEX WITH BUB1B; MAD2 AND CDC20.
RX PubMed=12163471; DOI=10.1083/jcb.200204048;
RA Chen R.H.;
RT "BubR1 is essential for kinetochore localization of other spindle
RT checkpoint proteins and its phosphorylation requires Mad1.";
RL J. Cell Biol. 158:487-496(2002).
RN [5]
RP FUNCTION, INTERACTION WITH BUB1 AND BUB1B, AND SUBCELLULAR LOCALIZATION.
RC TISSUE=Egg;
RX PubMed=12538762; DOI=10.1242/jcs.00255;
RA Campbell L., Hardwick K.G.;
RT "Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts.";
RL J. Cell Sci. 116:617-628(2003).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=15269280; DOI=10.1091/mbc.e04-01-0051;
RA Vigneron S., Prieto S., Bernis C., Labbe J.C., Castro A., Lorca T.;
RT "Kinetochore localization of spindle checkpoint proteins: who controls
RT whom?";
RL Mol. Biol. Cell 15:4584-4596(2004).
RN [7]
RP INTERACTION WITH ZNF207.
RX PubMed=24462186; DOI=10.1016/j.devcel.2013.12.013;
RA Jiang H., He X., Wang S., Jia J., Wan Y., Wang Y., Zeng R., Yates J. III,
RA Zhu X., Zheng Y.;
RT "A microtubule-associated zinc finger protein, BuGZ, regulates mitotic
RT chromosome alignment by ensuring Bub3 stability and kinetochore
RT targeting.";
RL Dev. Cell 28:268-281(2014).
CC -!- FUNCTION: Has a dual function in spindle-assembly checkpoint signaling
CC and in promoting the establishment of correct kinetochore-microtubule
CC (K-MT) attachments. Plays a role in the kinetochore-mediated mitotic
CC checkpoint, which delays anaphase onset until all chromosomes have
CC attached properly to the mitotic spindle. Required to establish and
CC maintain spindle checkpoint arrest in eggs.
CC {ECO:0000269|PubMed:12538762}.
CC -!- SUBUNIT: Found in complex with bub1 or bub1b (phosphorylated).
CC Component of a complex along with bub1b, mad2 and cdc20 located at the
CC kinetochore. Interacts with znf207/BuGZ. {ECO:0000269|PubMed:11231148,
CC ECO:0000269|PubMed:12538762, ECO:0000269|PubMed:24462186}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC Note=Aurora B controls the kinetochore localization. Kinetochore
CC localization starts in early prophase and maintains the localization
CC until the metaphase-anaphase transition. Diffusively localized to the
CC nucleus during interphase and telophase.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9YGY3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9YGY3-2; Sequence=VSP_044408;
CC -!- DEVELOPMENTAL STAGE: Maternal transcripts localize exclusively to
CC animal blastomeres. Active expression (mRNA level) in whole oocytes
CC during early stages (Dumont I-II). Transcripts accumulate in the animal
CC half during the latest stages of oogenesis (Dumont IV-VI). Localized in
CC the perinuclear cytoplasm in the full-grown oocyte. Close association
CC with chromosome movement in dividing blastomeres. After gastrulation
CC zygotic expression is observed in the whole ectodermal region, but
CC enhanced in the dorsoanterior region. In the tadpole, expression is
CC observed in the central nervous system, eyes and branchial arches.
CC {ECO:0000269|PubMed:10098604}.
CC -!- SIMILARITY: Belongs to the WD repeat BUB3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK12629.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB018419; BAA34999.1; -; mRNA.
DR EMBL; AF119790; AAK12629.1; ALT_INIT; mRNA.
DR EMBL; BC073086; AAH73086.1; -; mRNA.
DR RefSeq; NP_001083768.1; NM_001090299.1. [Q9YGY3-1]
DR AlphaFoldDB; Q9YGY3; -.
DR SMR; Q9YGY3; -.
DR BioGRID; 100431; 1.
DR IntAct; Q9YGY3; 1.
DR PRIDE; Q9YGY3; -.
DR DNASU; 399106; -.
DR GeneID; 399106; -.
DR KEGG; xla:399106; -.
DR CTD; 399106; -.
DR Xenbase; XB-GENE-955689; bub3.L.
DR OMA; WDPFNRK; -.
DR PRO; PR:Q9YGY3; -.
DR Proteomes; UP000186698; Chromosome 7L.
DR Bgee; 399106; Expressed in gastrula and 19 other tissues.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW Chromosome partition; Kinetochore; Meiosis; Mitosis; Nucleus;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..330
FT /note="Mitotic checkpoint protein BUB3"
FT /id="PRO_0000420122"
FT REPEAT 8..49
FT /note="WD 1"
FT REPEAT 52..89
FT /note="WD 2"
FT REPEAT 91..130
FT /note="WD 3"
FT REPEAT 133..169
FT /note="WD 4"
FT REPEAT 220..268
FT /note="WD 5"
FT VAR_SEQ 1..6
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_044408"
SQ SEQUENCE 330 AA; 37253 MW; 8075DC94738F5E19 CRC64;
MNTQTDMTGS NEFKLNQAPE DGISAVKFSP NTSQFLLVSS WDSSVRLYDV PANTMRLKYQ
HAGPVLDCAF YDPTHAWSGG LDHQLKMHDL NTDGDTVVGS HDAPIRCVEY CPEVNVIVTG
SWDQTVKLWD PRTPCNAGTF SQPDKVYTLS VSGDRLIVGT AGRRVLVWDL RNMGYVQQRR
ESSLKYQTRC IRAFPNKQGY VLSSIEGRVA VEYLDPSLEV QKKKYAFKCH RLKENNIEQI
YPVNAVSFHN LHNTFATGGS DGFVNIWDPF NKKRLCQFHR YPTSIASLAF SNDGSTLAIA
ASYMYEMDDI DHPEDAIYIR QVTDAETKPK
