ID   TRAP_STAAN              Reviewed;         167 AA.
AC   Q7A4W3;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Signal transduction protein TRAP;
DE   AltName: Full=Target of RNAIII-activating protein;
GN   Name=traP; OrderedLocusNames=SA1653;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RA   Stenz L.;
RT   "Shotgun proteomic analysis of total protein extract of S. aureus S30
RT   versus N315.";
RL   Submitted (NOV-2005) to UniProtKB.
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- FUNCTION: Signal transduction protein, which is a major regulator of
CC       staphylococcal pathogenesis. Phosphorylated TRAP leads to the
CC       activation of agr system and consequent RNAIII synthesis resulting in
CC       the expression of several virulence factors. Up-regulates the
CC       expression of most toxins and genes known to be necessary for biofilm
CC       formation (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane. Note=Membrane-associated.
CC       {ECO:0000250}.
CC   -!- PTM: Each of the three conserved histidine residues contributes to TRAP
CC       phosphorylation. Phosphorylation is essential for TRAP activity (By
CC       similarity). {ECO:0000250}.
CC   -!- PTM: Phosphorylation of TRAP is activated by RAP and necessary for the
CC       induction of RNAIII gene expression but not for ongoing transcription.
CC       TRAP is dephosphorylated from the mid-exponential phase of growth,
CC       which is when agr is activated and AIP is produced. RIP acts by
CC       inhibiting TRAP phosphorylation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAP family. {ECO:0000305}.
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DR   EMBL; BA000018; BAB42921.1; -; Genomic_DNA.
DR   PIR; B89970; B89970.
DR   RefSeq; WP_000737976.1; NC_002745.2.
DR   AlphaFoldDB; Q7A4W3; -.
DR   SMR; Q7A4W3; -.
DR   EnsemblBacteria; BAB42921; BAB42921; BAB42921.
DR   KEGG; sau:SA1653; -.
DR   HOGENOM; CLU_116220_0_0_9; -.
DR   OMA; YFGFANR; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007138; ABM_dom.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   SUPFAM; SSF54909; SSF54909; 1.
DR   PROSITE; PS51725; ABM; 1.
PE   1: Evidence at protein level;
KW   Membrane; Phosphoprotein; Virulence.
FT   CHAIN           1..167
FT                   /note="Signal transduction protein TRAP"
FT                   /id="PRO_0000289342"
FT   DOMAIN          67..158
FT                   /note="ABM"
FT   MOD_RES         66
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         79
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         154
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   167 AA;  19548 MW;  2BB731EEDBC00961 CRC64;
     MKKLYTSYGT YGFLHQIKIN NPTHQLFQFS ASDTSVIFEE TDGETVLKSP SIYEVIKEIG
     EFSEHHFYCA IFIPSTEDHA YQLEKKLISV DDNFRNFGGF KSYRLLRPAK GTTYKIYFGF
     ADRHAYEDFK QSDAFNDHFS KDALSHYFGS SGQHSSYFER YLYPIKE