ID   TRAP_STAAS              Reviewed;         167 AA.
AC   Q6G8A1;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Signal transduction protein TRAP;
DE   AltName: Full=Target of RNAIII-activating protein;
GN   Name=traP; OrderedLocusNames=SAS1756;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Signal transduction protein, which is a major regulator of
CC       staphylococcal pathogenesis. Phosphorylated TRAP leads to the
CC       activation of agr system and consequent RNAIII synthesis resulting in
CC       the expression of several virulence factors. Up-regulates the
CC       expression of most toxins and genes known to be necessary for biofilm
CC       formation (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane. Note=Membrane-associated.
CC       {ECO:0000250}.
CC   -!- PTM: Each of the three conserved histidine residues contributes to TRAP
CC       phosphorylation. Phosphorylation is essential for TRAP activity (By
CC       similarity). {ECO:0000250}.
CC   -!- PTM: Phosphorylation of TRAP is activated by RAP and necessary for the
CC       induction of RNAIII gene expression but not for ongoing transcription.
CC       TRAP is dephosphorylated from the mid-exponential phase of growth,
CC       which is when agr is activated and AIP is produced. RIP acts by
CC       inhibiting TRAP phosphorylation (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAP family. {ECO:0000305}.
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DR   EMBL; BX571857; CAG43560.1; -; Genomic_DNA.
DR   RefSeq; WP_000737978.1; NC_002953.3.
DR   AlphaFoldDB; Q6G8A1; -.
DR   SMR; Q6G8A1; -.
DR   KEGG; sas:SAS1756; -.
DR   HOGENOM; CLU_116220_0_0_9; -.
DR   OMA; YFGFANR; -.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007138; ABM_dom.
DR   InterPro; IPR011008; Dimeric_a/b-barrel.
DR   SUPFAM; SSF54909; SSF54909; 1.
DR   PROSITE; PS51725; ABM; 1.
PE   3: Inferred from homology;
KW   Membrane; Phosphoprotein; Virulence.
FT   CHAIN           1..167
FT                   /note="Signal transduction protein TRAP"
FT                   /id="PRO_0000289339"
FT   DOMAIN          67..158
FT                   /note="ABM"
FT   MOD_RES         66
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         79
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         154
FT                   /note="Phosphohistidine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   167 AA;  19563 MW;  9BB5F1F2E1755960 CRC64;
     MKKLYTSYGT YGFLHQIKIN NPTHQLFQFS ASDTSVIFEE TDGETVLKSP TKYDVIKEIG
     EFSEHHFYCA IFIPSTEDHA YQLEKKLISV DDNFRNFGGF KSYRLLRPAK GTTYKIYFGF
     ADRHAYEDFK QSDAFNDHFS KDALSHYFGS SGQHSSYFER YLYPIKE