TRAP_TYLCC
ID TRAP_TYLCC Reviewed; 134 AA.
AC Q9DXE6;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 29-SEP-2021, entry version 56.
DE RecName: Full=Transcriptional activator protein;
DE Short=TrAP;
DE AltName: Full=Protein C2;
DE AltName: Full=Protein L2;
GN ORFNames=C2, L2;
OS Tomato yellow leaf curl China virus (TYLCCNV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Begomovirus.
OX NCBI_TaxID=185793;
OH NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH NCBI_TaxID=185191; Sigesbeckia orientalis.
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate CHI;
RX PubMed=11682126; DOI=10.1016/s0168-1702(01)00363-x;
RA Yin Q., Yang H., Gong Q., Wang H., Liu Y., Hong Y., Tien P.;
RT "Tomato yellow leaf curl China virus: monopartite genome organization and
RT agroinfection of plants.";
RL Virus Res. 81:69-76(2001).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=11551077; DOI=10.1094/mpmi.2001.14.9.1125;
RA van Wezel R., Liu H., Tien P., Stanley J., Hong Y.;
RT "Gene C2 of the monopartite geminivirus tomato yellow leaf curl virus-China
RT encodes a pathogenicity determinant that is localized in the nucleus.";
RL Mol. Plant Microbe Interact. 14:1125-1128(2001).
RN [3]
RP FUNCTION, AND MUTAGENESIS OF CYS-36; CYS-38; CYS-46; THR-52; HIS-53;
RP HIS-58; CYS-59; SER-61; TYR-68 AND SER-74.
RX PubMed=11952122; DOI=10.1094/mpmi.2002.15.3.203;
RA van Wezel R., Dong X., Liu H., Tien P., Stanley J., Hong Y.;
RT "Mutation of three cysteine residues in Tomato yellow leaf curl virus-China
RT C2 protein causes dysfunction in pathogenesis and posttranscriptional gene-
RT silencing suppression.";
RL Mol. Plant Microbe Interact. 15:203-208(2002).
RN [4]
RP NUCLEAR LOCALIZATION SIGNAL.
RX PubMed=12768021; DOI=10.1128/jvi.77.12.7026-7033.2003;
RA Dong X., van Wezel R., Stanley J., Hong Y.;
RT "Functional characterization of the nuclear localization signal for a
RT suppressor of posttranscriptional gene silencing.";
RL J. Virol. 77:7026-7033(2003).
RN [5]
RP ZINC-BINDING.
RX PubMed=12477872; DOI=10.1128/jvi.77.1.696-700.2003;
RA Van Wezel R., Liu H., Wu Z., Stanley J., Hong Y.;
RT "Contribution of the zinc finger to zinc and DNA binding by a suppressor of
RT posttranscriptional gene silencing.";
RL J. Virol. 77:696-700(2003).
CC -!- FUNCTION: Strong activator of the late viral genes promoters. Acts as a
CC suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs. TrAP suppresses the
CC host RNA silencing by inhibiting adenosine kinase 2 (ADK2), a kinase
CC involved in a general methylation pathway. Also suppresses the host
CC basal defense by interacting with and inhibiting SNF1 kinase, a key
CC regulator of cell metabolism implicated in innate antiviral defense.
CC Determines pathogenicity (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:11952122}.
CC -!- SUBUNIT: Monomer. Homodimer. Homooligomer. Self-interaction correlates
CC with nuclear localization and efficient activation of transcription.
CC Monomers suppress local silencing by interacting with and inactivating
CC host adenosine kinase 2 (ADK2) in the cytoplasm. Interacts with and
CC inhibits host SNF1 kinase. Binds to ssDNA (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:11551077}. Host
CC cytoplasm {ECO:0000250}. Note=The phosphorylated form appears to
CC accumulate almost exclusively in the nucleus, whereas the non-
CC phosphorylated form is found in both nucleus and cytoplasm.
CC {ECO:0000250}.
CC -!- DOMAIN: The zinc finger and the transactivation region are involved in
CC PTGS suppression. {ECO:0000250}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the geminiviridae transcriptional activator
CC protein family. {ECO:0000305}.
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DR EMBL; AF311734; AAG27474.1; -; Genomic_DNA.
DR RefSeq; NP_660167.1; NC_004044.1.
DR GeneID; 949227; -.
DR KEGG; vg:949227; -.
DR Proteomes; UP000008267; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR000942; Gemini_AL2.
DR Pfam; PF01440; Gemini_AL2; 1.
DR PRINTS; PR00230; GEMCOATAL2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Host cytoplasm; Host nucleus;
KW Host-virus interaction; Metal-binding; Phosphoprotein; Reference proteome;
KW Suppressor of RNA silencing; Zinc; Zinc-finger.
FT CHAIN 1..134
FT /note="Transcriptional activator protein"
FT /id="PRO_0000312153"
FT ZN_FING 36..53
FT REGION 73..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..134
FT /note="Transactivation"
FT /evidence="ECO:0000250"
FT MOTIF 17..31
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:12768021"
FT MUTAGEN 36
FT /note="C->R: Loss of suppression of PTGS. Produces
FT chlorosis and mosaic symptoms."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 38
FT /note="C->N: Loss of suppression of PTGS. Produces
FT chlorosis and mosaic symptoms."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 46
FT /note="C->I: Loss of suppression of PTGS. Produces
FT chlorosis and mosaic symptoms."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 52
FT /note="T->M: Produces local necrotic ringspots and systemic
FT necrosis in N.benthamiana plants."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 53
FT /note="H->P: Produced irregular necrotic lesions on
FT inoculated leaves."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 58
FT /note="H->S: Produces local necrotic ringspots and systemic
FT necrosis in N.benthamiana plants."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 59
FT /note="C->S: Produces local necrotic ringspots and systemic
FT necrosis in N.benthamiana plants."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 61
FT /note="S->R: Produces local necrotic ringspots and systemic
FT necrosis in N.benthamiana plants."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 68
FT /note="Y->D: Produced irregular necrotic lesions on
FT inoculated leaves."
FT /evidence="ECO:0000269|PubMed:11952122"
FT MUTAGEN 74
FT /note="S->D: Produces local necrotic ringspots and systemic
FT necrosis in N.benthamiana plants."
FT /evidence="ECO:0000269|PubMed:11952122"
SQ SEQUENCE 134 AA; 15178 MW; 661BCEC6B8F09049 CRC64;
MRSSSPSTGH STQVPIKVQH RIAKKTTRRR RVDLPCGCSY FVALGCHNHG FTHRGTTHCS
SIREWRVYLD GQKSPVFQDN QTPRETISEE PRHNHNTSPI QLQPEESVGD TQMFSNLPNL
DSFTSSDLAF LKSI
