BUBR1_ARATH
ID BUBR1_ARATH Reviewed; 395 AA.
AC O22806; F4IFV2; Q8L9V1;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Mitotic spindle checkpoint protein BUBR1;
DE AltName: Full=BUB1-related protein 1;
GN Name=BUBR1; Synonyms=MAD3; OrderedLocusNames=At2g33560; ORFNames=F4P9.33;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-233 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=16009969; DOI=10.1074/mcp.m500007-mcp200;
RA Feilner T., Hultschig C., Lee J., Meyer S., Immink R.G.H., Koenig A.,
RA Possling A., Seitz H., Beveridge A., Scheel D., Cahill D.J., Lehrach H.,
RA Kreutzberger J., Kersten B.;
RT "High throughput identification of potential Arabidopsis mitogen-activated
RT protein kinases substrates.";
RL Mol. Cell. Proteomics 4:1558-1568(2005).
RN [5]
RP FUNCTION, INTERACTION WITH MAD2 AND BUB3.1, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=19710914; DOI=10.1371/journal.pone.0006757;
RA Caillaud M.-C., Paganelli L., Lecomte P., Deslandes L., Quentin M.,
RA Pecrix Y., Le Bris M., Marfaing N., Abad P., Favery B.;
RT "Spindle assembly checkpoint protein dynamics reveal conserved and
RT unsuspected roles in plant cell division.";
RL PLoS ONE 4:E6757-E6757(2009).
RN [6]
RP INTERACTION WITH CDC20-1 AND CDC20-2.
RX PubMed=21687678; DOI=10.1371/journal.pone.0020618;
RA Kevei Z., Baloban M., Da Ines O., Tiricz H., Kroll A., Regulski K.,
RA Mergaert P., Kondorosi E.;
RT "Conserved CDC20 cell cycle functions are carried out by two of the five
RT isoforms in Arabidopsis thaliana.";
RL PLoS ONE 6:E20618-E20618(2011).
CC -!- FUNCTION: Essential component of the mitotic checkpoint. Required for
CC normal mitosis progression. The mitotic checkpoint delays anaphase
CC until all chromosomes are properly attached to the mitotic spindle. One
CC of its checkpoint functions may be to inhibit the activity of the
CC anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of
CC CDC20 to APC/C (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:19710914}.
CC -!- SUBUNIT: Interacts with anaphase-promoting complex/cyclosome (APC/C)
CC (By similarity). Part of the mitotic checkpoint complex (MCC);
CC interacts with CDC20-1 and CDC20-2. Interacts with MAD2 at
CC chromocenters and with BUB3.1. {ECO:0000250,
CC ECO:0000269|PubMed:19710914, ECO:0000269|PubMed:21687678}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:19710914}.
CC Cytoplasm {ECO:0000269|PubMed:19710914}. Nucleus
CC {ECO:0000269|PubMed:19710914}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:19710914}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:19710914}. Cytoplasm, cytoskeleton, microtubule
CC organizing center {ECO:0000250|UniProtKB:O60566}. Note=Cytoplasmic in
CC interphase cells. Accumulates onto both kinetochores and the spindle
CC microtubules in cell arrested in metaphase. Associates with unattached
CC kinetochores in early prophase upon spindle assembly checkpoint (SAC)
CC activation. Present in chromocenters.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O22806-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O22806-2; Sequence=VSP_047765;
CC -!- TISSUE SPECIFICITY: Expressed in actively dividing tissues, early in
CC organ development, in young leaves, lateral root primordia and root
CC meristems. {ECO:0000269|PubMed:19710914}.
CC -!- INDUCTION: Cell cycle regulated expression with a distinct expression
CC peak at the G2/M boundary. {ECO:0000269|PubMed:19710914}.
CC -!- DOMAIN: The KEN box is required for its ubiquitination and degradation.
CC {ECO:0000250}.
CC -!- DOMAIN: BUB1 N-terminal domain directs kinetochore localization and
CC binding to BUB3.
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DR EMBL; AC002332; AAB80673.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08852.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08853.1; -; Genomic_DNA.
DR EMBL; AY088206; AAM65748.1; -; mRNA.
DR EMBL; CK118048; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A84747; A84747.
DR RefSeq; NP_001189668.1; NM_001202739.2. [O22806-2]
DR RefSeq; NP_565767.1; NM_128916.3. [O22806-1]
DR AlphaFoldDB; O22806; -.
DR SMR; O22806; -.
DR BioGRID; 3268; 6.
DR IntAct; O22806; 2.
DR STRING; 3702.AT2G33560.2; -.
DR PaxDb; O22806; -.
DR PRIDE; O22806; -.
DR ProteomicsDB; 239103; -. [O22806-1]
DR EnsemblPlants; AT2G33560.1; AT2G33560.1; AT2G33560. [O22806-1]
DR EnsemblPlants; AT2G33560.2; AT2G33560.2; AT2G33560. [O22806-2]
DR GeneID; 817921; -.
DR Gramene; AT2G33560.1; AT2G33560.1; AT2G33560. [O22806-1]
DR Gramene; AT2G33560.2; AT2G33560.2; AT2G33560. [O22806-2]
DR KEGG; ath:AT2G33560; -.
DR Araport; AT2G33560; -.
DR TAIR; locus:2050977; AT2G33560.
DR eggNOG; KOG1166; Eukaryota.
DR OMA; PPTMTPC; -.
DR PhylomeDB; O22806; -.
DR PRO; PR:O22806; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22806; baseline and differential.
DR Genevisible; O22806; AT.
DR GO; GO:0010369; C:chromocenter; IDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0000776; C:kinetochore; IDA:TAIR.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005876; C:spindle microtubule; IDA:UniProtKB.
DR GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR GO; GO:0051754; P:meiotic sister chromatid cohesion, centromeric; IBA:GO_Central.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IDA:TAIR.
DR InterPro; IPR015661; Bub1/Mad3.
DR InterPro; IPR013212; Mad3/Bub1_I.
DR PANTHER; PTHR14030; PTHR14030; 1.
DR Pfam; PF08311; Mad3_BUB1_I; 1.
DR SMART; SM00777; Mad3_BUB1_I; 1.
DR PROSITE; PS51489; BUB1_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Centromere; Chromosome; Cytoplasm;
KW Cytoskeleton; Kinetochore; Nucleus; Reference proteome.
FT CHAIN 1..395
FT /note="Mitotic spindle checkpoint protein BUBR1"
FT /id="PRO_0000423379"
FT DOMAIN 69..224
FT /note="BUB1 N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00822"
FT REGION 59..206
FT /note="Necessary for kinetochore localization"
FT /evidence="ECO:0000250"
FT REGION 210..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 342..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 118..125
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT MOTIF 219..221
FT /note="KEN box 1"
FT /evidence="ECO:0000250"
FT MOTIF 304..306
FT /note="KEN box 2"
FT /evidence="ECO:0000250"
FT COMPBIAS 224..263
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 216
FT /note="E -> EQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047765"
FT CONFLICT 333
FT /note="S -> F (in Ref. 3; AAM65748)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 395 AA; 45374 MW; 8E2C2389AE272414 CRC64;
MAAETKVQVS DPEAEFLNSK QETGYEWELF KENVRPLKRG RNVGILNHAL KSHSDHQLRK
NLIEKRRNLI EAIDEYEGDD PLSPWIECIK WVQEAFPPGG ECSGLLVIYE QCVRKFWHSE
RYKDDLRYLK VWLEYAEHCA DAEVIYKFLE VNEIGKTHAV YYIAYALHIE FKNKVKTANE
IFNLGISRDA KPVEKLNDAY KKFMVRTMRR SNTADEEPKE NNDLPSRSFG TLLSRGDNNA
RRQALGSSNP QAKKLKPNQS SKTPFAIYAD AVSDTTSGNQ PESDKSRPEF GSWLMLGGRA
ERNKENNSLP RKWASFKVPQ KPIVRTVAAA SASTFEVFVD EEECTEEEEE KKKNDETISS
SSNVLPLNGG REIKKETELL RQNPLRHFPP NSFLR
