ID   TRAR_RHIRD              Reviewed;         234 AA.
AC   P33905;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Transcriptional activator protein TraR;
GN   Name=traR;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A348;
RX   PubMed=8188582; DOI=10.1128/jb.176.10.2796-2806.1994;
RA   Fuqua W.C. Jr., Winans S.C.;
RT   "A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid
RT   conjugal transfer in the presence of a plant tumor metabolite.";
RL   J. Bacteriol. 176:2796-2806(1994).
RN   [2]
RP   SEQUENCE REVISION.
RA   Winans S.C.;
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Positive regulation of conjugal transfer of Ti plasmids. TraR
CC       activates target genes in the presence of AAI and also activates traR
CC       and traI themselves.
CC   -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional
CC       regulatory protein family. {ECO:0000305}.
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DR   EMBL; L08596; AAA64793.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AF242881; AAC28121.1; -; Genomic_DNA.
DR   RefSeq; NP_059701.1; NC_002377.1.
DR   RefSeq; WP_010892389.1; NZ_QSNU01000012.1.
DR   PDB; 1H0M; X-ray; 3.00 A; A/B/C/D=1-234.
DR   PDB; 1L3L; X-ray; 1.66 A; A/B/C/D=1-234.
DR   PDBsum; 1H0M; -.
DR   PDBsum; 1L3L; -.
DR   AlphaFoldDB; P33905; -.
DR   SMR; P33905; -.
DR   BindingDB; P33905; -.
DR   ChEMBL; CHEMBL5865; -.
DR   DrugBank; DB08081; 3-OXO-OCTANOIC ACID (2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE.
DR   OrthoDB; 1687028at2; -.
DR   EvolutionaryTrace; P33905; -.
DR   PRO; PR:P33905; -.
DR   CollecTF; EXPREG_00000a40; -.
DR   GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   DisProt; DP00198; -.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.80; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR   InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03472; Autoind_bind; 1.
DR   Pfam; PF00196; GerE; 1.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF75516; SSF75516; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Conjugation; DNA-binding; Plasmid; Quorum sensing;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..234
FT                   /note="Transcriptional activator protein TraR"
FT                   /id="PRO_0000184192"
FT   DOMAIN          167..232
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        191..210
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   HELIX           4..11
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           18..31
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          35..43
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          46..51
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           55..63
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           66..68
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           71..78
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          83..86
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           87..90
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   TURN            91..93
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           96..106
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   TURN            107..109
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          111..119
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           121..123
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          125..136
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           145..161
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   STRAND          167..169
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           176..185
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   TURN            186..188
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           191..198
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           202..216
FT                   /evidence="ECO:0007829|PDB:1L3L"
FT   HELIX           221..230
FT                   /evidence="ECO:0007829|PDB:1L3L"
SQ   SEQUENCE   234 AA;  26734 MW;  1B0AEC296F1521A5 CRC64;
     MQHWLDKLTD LAAIEGDECI LKTGLADIAD HFGFTGYAYL HIQHRHITAV TNYHRQWQST
     YFDKKFEALD PVVKRARSRK HIFTWSGEHE RPTLSKDERA FYDHASDFGI RSGITIPIKT
     ANGFMSMFTM ASDKPVIDLD REIDAVAAAA TIGQIHARIS FLRTTPTAED AAWLDPKEAT
     YLRWIAVGKT MEEIADVEGV KYNSVRVKLR EAMKRFDVRS KAHLTALAIR RKLI