ID   TRAT1_ECOLI             Reviewed;         244 AA.
AC   P13979; P07177;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=TraT complement resistance protein;
DE   Flags: Precursor;
GN   Name=traT; OrderedLocusNames=ECOK12F101;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=3323526; DOI=10.1016/0022-2836(87)90452-9;
RA   Jalajakumari M.B., Guidolin A., Buhj H.J., Manning P.A.;
RT   "Surface exclusion genes traS and traT of the F sex factor of Escherichia
RT   coli K-12. Determination of the nucleotide sequence and promoter and
RT   terminator activities.";
RL   J. Mol. Biol. 198:1-11(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 221-244.
RC   STRAIN=K12;
RX   PubMed=2680768; DOI=10.1016/0378-1119(89)90179-0;
RA   Jalajakumari M.B., Manning P.A.;
RT   "Nucleotide sequence of the traD region in the Escherichia coli F sex
RT   factor.";
RL   Gene 81:195-202(1989).
CC   -!- FUNCTION: Responsible for preventing unproductive conjugation between
CC       bacteria carrying like plasmids.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor.
CC   -!- SIMILARITY: Belongs to the TraT lipoprotein family. {ECO:0000305}.
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DR   EMBL; X06915; CAA30012.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44197.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97971.1; -; Genomic_DNA.
DR   EMBL; M29254; AAA83927.1; -; Genomic_DNA.
DR   PIR; S01757; S01757.
DR   RefSeq; NP_061480.1; NC_002483.1.
DR   RefSeq; WP_000978374.1; NC_002483.1.
DR   AlphaFoldDB; P13979; -.
DR   PRIDE; P13979; -.
DR   PhylomeDB; P13979; -.
DR   PRO; PR:P13979; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008874; TraT_complement-R.
DR   Pfam; PF05818; TraT; 1.
DR   PIRSF; PIRSF002859; Lipo_traT; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Conjugation; Lipoprotein; Membrane; Palmitate;
KW   Plasmid; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           22..244
FT                   /note="TraT complement resistance protein"
FT                   /id="PRO_0000018204"
FT   LIPID           22
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           22
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CONFLICT        141
FT                   /note="A -> G (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   244 AA;  26032 MW;  A5921224AAE8D101 CRC64;
     MMKTKKLMMV ALVSSTLALS GCGAMSTAIK KRNLEVKTQM SETIWLEPAS ERTVFLQIKN
     TSDKDMSGLQ GKIADAVKAK GYQVVTSPDK AYYWIQANVL KADKMDLRES QGWLNRGYEG
     AAVGAALGAG ITGYNSNSAG ATLGVGLAAG LVGMAADAMV EDVNYTMITD VQIAERTKAT
     VTTDNVAALR QGTSGAKIQT STETGNQHKY QTRVVSNANK VNLKFEEAKP VLEDQLAKSI
     ANIL