TRAT1_HUMAN
ID TRAT1_HUMAN Reviewed; 186 AA.
AC Q6PIZ9; Q9NZX5;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=T-cell receptor-associated transmembrane adapter 1;
DE AltName: Full=T-cell receptor-interacting molecule;
DE Short=TRIM;
DE AltName: Full=pp29/30;
GN Name=TRAT1; Synonyms=TCRIM; ORFNames=HSPC062;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 152-167, IDENTIFICATION BY
RP MASS SPECTROMETRY, DIMERIZATION, INTERACTION WITH THE TCR COMPLEX AND
RP PIK3R1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION AT TYR-79,
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF TYR-79.
RX PubMed=9687533; DOI=10.1084/jem.188.3.561;
RA Bruyns E., Marie-Cardine A., Kirchgessner H., Sagolla K., Shevchenko A.,
RA Mann M., Autschbach F., Bensussan A., Meuer S., Schraven B.;
RT "T cell receptor (TCR) interacting molecule (TRIM), a novel disulfide-
RT linked dimer associated with the TCR-CD3-zeta complex, recruits
RT intracellular signaling proteins to the plasma membrane.";
RL J. Exp. Med. 188:561-575(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for 300
RT previously undefined genes expressed in CD34+ hematopoietic stem/progenitor
RT cells.";
RL Genome Res. 10:1546-1560(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH CD3Z.
RX PubMed=11390434; DOI=10.1084/jem.193.11.1269;
RA Kirchgessner H., Dietrich J., Scherer J., Isomaeki P., Korinek V.,
RA Hilgert I., Bruyns E., Leo A., Cope A.P., Schraven B.;
RT "The transmembrane adaptor protein TRIM regulates T-cell receptor (TCR)
RT expression and TCR-mediated signaling via an association with the TCR zeta
RT chain.";
RL J. Exp. Med. 193:1269-1284(2001).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=16160011; DOI=10.1182/blood-2005-06-2273;
RA Tedoldi S., Paterson J.C., Hansmann M.-L., Natkunam Y., Rudiger T.,
RA Angelisova P., Du M.Q., Roberton H., Roncador G., Sanchez L., Pozzobon M.,
RA Masir N., Barry R., Pileri S., Mason D.Y., Marafioti T., Horejsi V.;
RT "Transmembrane adaptor molecules: a new category of lymphoid-cell
RT markers.";
RL Blood 107:213-221(2006).
CC -!- FUNCTION: Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at
CC the surface of T-cells. {ECO:0000269|PubMed:11390434}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with CD3Z. When
CC phosphorylated, interacts with PIK3R1. {ECO:0000269|PubMed:11390434,
CC ECO:0000269|PubMed:9687533}.
CC -!- INTERACTION:
CC Q6PIZ9; Q96IW7: SEC22A; NbExp=3; IntAct=EBI-17710254, EBI-8652744;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9687533};
CC Single-pass type III membrane protein {ECO:0000269|PubMed:9687533}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in thymus, and to a lesser
CC extent in spleen, lymph node and peripheral blood lymphocytes. Present
CC in T-cells and NK cells, but not B-cells (at protein level).
CC {ECO:0000269|PubMed:16160011, ECO:0000269|PubMed:9687533}.
CC -!- DEVELOPMENTAL STAGE: Strongly expressed in fetal thymus at weeks 17-24
CC of gestation. Undetectable in bone marrow and fetal liver.
CC {ECO:0000269|PubMed:9687533}.
CC -!- PTM: Phosphorylated on tyrosines by LCK or FYN upon TCR activation.
CC {ECO:0000269|PubMed:9687533}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF29034.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF161547; AAF29034.1; ALT_FRAME; mRNA.
DR EMBL; BC025713; AAH25713.1; -; mRNA.
DR CCDS; CCDS33813.1; -.
DR RefSeq; NP_001304676.1; NM_001317747.1.
DR RefSeq; NP_057472.2; NM_016388.3.
DR AlphaFoldDB; Q6PIZ9; -.
DR SMR; Q6PIZ9; -.
DR BioGRID; 119154; 23.
DR IntAct; Q6PIZ9; 2.
DR MINT; Q6PIZ9; -.
DR STRING; 9606.ENSP00000295756; -.
DR iPTMnet; Q6PIZ9; -.
DR PhosphoSitePlus; Q6PIZ9; -.
DR BioMuta; TRAT1; -.
DR DMDM; 74749223; -.
DR MassIVE; Q6PIZ9; -.
DR MaxQB; Q6PIZ9; -.
DR PaxDb; Q6PIZ9; -.
DR PeptideAtlas; Q6PIZ9; -.
DR PRIDE; Q6PIZ9; -.
DR ProteomicsDB; 67189; -.
DR Antibodypedia; 1192; 262 antibodies from 31 providers.
DR DNASU; 50852; -.
DR Ensembl; ENST00000295756.11; ENSP00000295756.6; ENSG00000163519.14.
DR GeneID; 50852; -.
DR KEGG; hsa:50852; -.
DR MANE-Select; ENST00000295756.11; ENSP00000295756.6; NM_016388.4; NP_057472.2.
DR UCSC; uc003dxi.2; human.
DR CTD; 50852; -.
DR DisGeNET; 50852; -.
DR GeneCards; TRAT1; -.
DR HGNC; HGNC:30698; TRAT1.
DR HPA; ENSG00000163519; Tissue enriched (lymphoid).
DR MIM; 604962; gene.
DR neXtProt; NX_Q6PIZ9; -.
DR OpenTargets; ENSG00000163519; -.
DR PharmGKB; PA128394655; -.
DR VEuPathDB; HostDB:ENSG00000163519; -.
DR eggNOG; ENOG502S7P1; Eukaryota.
DR GeneTree; ENSGT00390000004910; -.
DR HOGENOM; CLU_125033_1_0_1; -.
DR InParanoid; Q6PIZ9; -.
DR OMA; VWAILAF; -.
DR OrthoDB; 1572760at2759; -.
DR PhylomeDB; Q6PIZ9; -.
DR TreeFam; TF336066; -.
DR PathwayCommons; Q6PIZ9; -.
DR Reactome; R-HSA-1257604; PIP3 activates AKT signaling.
DR Reactome; R-HSA-202424; Downstream TCR signaling.
DR Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer.
DR Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR SignaLink; Q6PIZ9; -.
DR BioGRID-ORCS; 50852; 13 hits in 1055 CRISPR screens.
DR GeneWiki; TRAT1; -.
DR GenomeRNAi; 50852; -.
DR Pharos; Q6PIZ9; Tbio.
DR PRO; PR:Q6PIZ9; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q6PIZ9; protein.
DR Bgee; ENSG00000163519; Expressed in buccal mucosa cell and 104 other tissues.
DR ExpressionAtlas; Q6PIZ9; baseline and differential.
DR Genevisible; Q6PIZ9; HS.
DR GO; GO:0034451; C:centriolar satellite; IDA:HPA.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0072686; C:mitotic spindle; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0042101; C:T cell receptor complex; IDA:MGI.
DR GO; GO:0005068; F:transmembrane receptor protein tyrosine kinase adaptor activity; TAS:ProtInc.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0006968; P:cellular defense response; TAS:ProtInc.
DR GO; GO:0001920; P:negative regulation of receptor recycling; IDA:MGI.
DR GO; GO:0051051; P:negative regulation of transport; IDA:MGI.
DR GO; GO:0050850; P:positive regulation of calcium-mediated signaling; IDA:MGI.
DR GO; GO:0050862; P:positive regulation of T cell receptor signaling pathway; IDA:MGI.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR InterPro; IPR020399; T-cell_rcpt-assoc_TM_adapter-1.
DR PANTHER; PTHR15951; PTHR15951; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Cell membrane; Direct protein sequencing;
KW Disulfide bond; Immunity; Membrane; Phosphoprotein; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..186
FT /note="T-cell receptor-associated transmembrane adapter 1"
FT /id="PRO_0000083344"
FT TOPO_DOM 1..7
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Signal-anchor for type III membrane
FT protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 79..82
FT /note="Interaction with PIK3R1"
FT /evidence="ECO:0000269|PubMed:9687533"
FT REGION 116..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3UU67"
FT MOD_RES 79
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000305|PubMed:9687533"
FT DISULFID 7
FT /note="Interchain"
FT /evidence="ECO:0000305"
FT VARIANT 137
FT /note="D -> G (in dbSNP:rs57744779)"
FT /id="VAR_062195"
FT VARIANT 148
FT /note="S -> P (in dbSNP:rs61585973)"
FT /id="VAR_062196"
FT MUTAGEN 79
FT /note="Y->F: Abolishes interaction with PIK3R1."
FT /evidence="ECO:0000269|PubMed:9687533"
SQ SEQUENCE 186 AA; 21211 MW; 236A4BEFF427EA3E CRC64;
MSGISGCPFF LWGLLALLGL ALVISLIFNI SHYVEKQRQD KMYSYSSDHT RVDEYYIEDT
PIYGNLDDMI SEPMDENCYE QMKARPEKSV NKMQEATPSA QATNETQMCY ASLDHSVKGK
RRKPRKQNTH FSDKDGDEQL HAIDASVSKT TLVDSFSPES QAVEENIHDD PIRLFGLIRA
KREPIN
