BUCC_APLCA
ID BUCC_APLCA Reviewed; 505 AA.
AC P20481; Q9TWM2;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Buccalin;
DE Contains:
DE RecName: Full=Buccalin-D;
DE Contains:
DE RecName: Full=Buccalin-E;
DE Contains:
DE RecName: Full=Buccalin-F;
DE Contains:
DE RecName: Full=Buccalin-G;
DE Contains:
DE RecName: Full=Buccalin-H;
DE Contains:
DE RecName: Full=Buccalin-A;
DE Contains:
DE RecName: Full=Buccalin-I;
DE Contains:
DE RecName: Full=Buccalin-J;
DE Contains:
DE RecName: Full=Buccalin-K;
DE Contains:
DE RecName: Full=Buccalin-L;
DE Contains:
DE RecName: Full=Buccalin-B;
DE Short=BUCb;
DE Contains:
DE RecName: Full=Buccalin-M;
DE Contains:
DE RecName: Full=Buccalin gene-predicted acidic peptide A;
DE Short=BGPAP A;
DE Contains:
DE RecName: Full=Buccalin-N;
DE Contains:
DE RecName: Full=Buccalin-O;
DE Contains:
DE RecName: Full=Buccalin-P;
DE Contains:
DE RecName: Full=Buccalin-Q;
DE Contains:
DE RecName: Full=Buccalin-R;
DE Contains:
DE RecName: Full=Buccalin-C;
DE Contains:
DE RecName: Full=Buccalin-S;
DE Contains:
DE RecName: Full=Buccalin gene-predicted acidic peptide B;
DE Short=BGPAP B;
DE Flags: Precursor;
OS Aplysia californica (California sea hare).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Heterobranchia; Euthyneura; Tectipleura; Aplysiida; Aplysioidea;
OC Aplysiidae; Aplysia.
OX NCBI_TaxID=6500;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 437-447 AND 451-461, AND
RP AMIDATION AT VAL-74; LEU-88; LEU-102; ILE-116; LEU-129; LEU-143; LEU-157;
RP LEU-171; LEU-185; LEU-199; LEU-213; LEU-227; LEU-241; LEU-254; LEU-267;
RP LEU-281; LEU-294; LEU-307; LEU-321; LEU-335; GLU-349; LEU-363; LEU-377;
RP LEU-391; LEU-405; LEU-419; LEU-433; ILE-447; ILE-461 AND LEU-475.
RX PubMed=8101868; DOI=10.1523/jneurosci.13-08-03346.1993;
RA Miller M.W., Beushausen S., Cropper E.C., Eisinger K., Stamm S.,
RA Vilim F.S., Vitek A., Zajc A., Kupfermann I., Brosius J., Weiss K.R.;
RT "The buccalin-related neuropeptides: isolation and characterization of an
RT Aplysia cDNA clone encoding a family of peptide cotransmitters.";
RL J. Neurosci. 13:3346-3357(1993).
RN [2]
RP PROTEIN SEQUENCE OF 133-143; 147-157; 161-171; 175-185; 189-199 AND
RP 203-213, AND AMIDATION AT LEU-143; LEU-157; LEU-171; LEU-185; LEU-199 AND
RP LEU-213.
RX PubMed=3413086; DOI=10.1073/pnas.85.16.6177;
RA Cropper E.C., Miller M.W., Tenenbaum R., Kolks M.A.G., Kupfermann I.,
RA Weiss K.R.;
RT "Structure and action of buccalin: a modulatory neuropeptide localized to
RT an identified small cardioactive peptide-containing cholinergic motor
RT neuron of Aplysia californica.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:6177-6181(1988).
RN [3]
RP PROTEIN SEQUENCE OF 367-377, AND FUNCTION.
RX PubMed=7991459; DOI=10.1016/0196-9781(94)90058-2;
RA Vilim F.S., Cropper E.C., Rosen S.C., Tenenbaum R., Kupfermann I.,
RA Weiss K.R.;
RT "Structure, localization, and action of buccalin B: a bioactive peptide
RT from Aplysia.";
RL Peptides 15:959-969(1994).
RN [4]
RP MASS SPECTROMETRY.
RX PubMed=9809658; DOI=10.1016/s0196-9781(98)00094-1;
RA Li L., Moroz T.P., Garden R.W., Floyd P.D., Weiss K.R., Sweedler J.V.;
RT "Mass spectrometric survey of interganglionically transported peptides in
RT Aplysia.";
RL Peptides 19:1425-1433(1998).
CC -!- FUNCTION: Modulatory neuropeptide, acting presynaptically on nerve
CC terminals to inhibit acetylcholine release.
CC {ECO:0000269|PubMed:7991459}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Cholinergic motor neuron B15 innervating buccal
CC muscles in Aplysia.
CC -!- MASS SPECTROMETRY: [Buccalin-F]: Mass=1059.1; Mass_error=0.4;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-G]: Mass=1101.6; Mass_error=0.0;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-A]: Mass=1052.8; Mass_error=0.3;
CC Method=MALDI; Note=Buccalin-A.; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-L]: Mass=1053.6; Mass_error=0.1;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-B]: Mass=1151.9; Mass_error=0.3;
CC Method=MALDI; Note=Buccalin-B.; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-M]: Mass=1150.6; Mass_error=0.1;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-N]: Mass=1112.9; Mass_error=0.4;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-Q]: Mass=1251.6; Mass_error=0.0;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-R]: Mass=1096.7; Mass_error=0.2;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-C]: Mass=1168.7; Mass_error=0.2;
CC Method=MALDI; Note=Buccalin-C.; Evidence={ECO:0000269|PubMed:9809658};
CC -!- MASS SPECTROMETRY: [Buccalin-S]: Mass=1274.9; Mass_error=0.2;
CC Method=MALDI; Evidence={ECO:0000269|PubMed:9809658};
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DR EMBL; S64298; AAB27696.2; -; mRNA.
DR PIR; A35594; A35594.
DR RefSeq; NP_001191649.1; NM_001204720.1.
DR AlphaFoldDB; P20481; -.
DR PRIDE; P20481; -.
DR GeneID; 100533443; -.
DR OrthoDB; 1780047at2759; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Pyrrolidone carboxylic acid; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PROPEP 26..62
FT /evidence="ECO:0000255"
FT /id="PRO_0000001881"
FT PEPTIDE 63..74
FT /note="Buccalin-D"
FT /id="PRO_0000001882"
FT PEPTIDE 78..88
FT /note="Buccalin-E"
FT /id="PRO_0000001883"
FT PEPTIDE 93..102
FT /note="Buccalin-F"
FT /id="PRO_0000001884"
FT PEPTIDE 106..116
FT /note="Buccalin-G"
FT /id="PRO_0000001885"
FT PEPTIDE 120..129
FT /note="Buccalin-H"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001886"
FT PEPTIDE 133..143
FT /note="Buccalin-A"
FT /id="PRO_0000001887"
FT PEPTIDE 147..157
FT /note="Buccalin-A"
FT /id="PRO_0000001888"
FT PEPTIDE 161..171
FT /note="Buccalin-A"
FT /id="PRO_0000001889"
FT PEPTIDE 175..185
FT /note="Buccalin-A"
FT /id="PRO_0000001890"
FT PEPTIDE 189..199
FT /note="Buccalin-A"
FT /id="PRO_0000001891"
FT PEPTIDE 203..213
FT /note="Buccalin-A"
FT /id="PRO_0000001892"
FT PEPTIDE 217..227
FT /note="Buccalin-I"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001893"
FT PEPTIDE 231..241
FT /note="Buccalin-J"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001894"
FT PEPTIDE 245..254
FT /note="Buccalin-K"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001895"
FT PEPTIDE 258..267
FT /note="Buccalin-L"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001896"
FT PEPTIDE 271..281
FT /note="Buccalin-J"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001897"
FT PEPTIDE 285..294
FT /note="Buccalin-L"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001898"
FT PEPTIDE 298..307
FT /note="Buccalin-L"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001899"
FT PEPTIDE 311..321
FT /note="Buccalin-B"
FT /id="PRO_0000001900"
FT PEPTIDE 325..335
FT /note="Buccalin-M"
FT /id="PRO_0000001901"
FT PEPTIDE 339..349
FT /note="Buccalin gene-predicted acidic peptide A"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001902"
FT PEPTIDE 353..363
FT /note="Buccalin-N"
FT /id="PRO_0000001903"
FT PEPTIDE 367..377
FT /note="Buccalin-B"
FT /id="PRO_0000001904"
FT PEPTIDE 381..391
FT /note="Buccalin-O"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001905"
FT PEPTIDE 396..405
FT /note="Buccalin-P"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001906"
FT PEPTIDE 409..419
FT /note="Buccalin-Q"
FT /id="PRO_0000001907"
FT PEPTIDE 424..433
FT /note="Buccalin-R"
FT /id="PRO_0000001908"
FT PEPTIDE 437..447
FT /note="Buccalin-C"
FT /id="PRO_0000001909"
FT PEPTIDE 451..461
FT /note="Buccalin-C"
FT /id="PRO_0000001910"
FT PEPTIDE 465..475
FT /note="Buccalin-S"
FT /id="PRO_0000001911"
FT PEPTIDE 479..492
FT /note="Buccalin gene-predicted acidic peptide B"
FT /evidence="ECO:0000255"
FT /id="PRO_0000001912"
FT PROPEP 495..505
FT /evidence="ECO:0000255"
FT /id="PRO_0000001913"
FT REGION 472..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..505
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 74
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 88
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 102
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 106
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000255"
FT MOD_RES 116
FT /note="Isoleucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 129
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 143
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 157
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 171
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 185
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 199
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 213
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:3413086,
FT ECO:0000269|PubMed:8101868"
FT MOD_RES 227
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 241
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 254
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 267
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 281
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 294
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 307
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 321
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 335
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 349
FT /note="Glutamic acid 1-amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 363
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 377
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 391
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 405
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 419
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 433
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 447
FT /note="Isoleucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 461
FT /note="Isoleucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
FT MOD_RES 465
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000255"
FT MOD_RES 475
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:8101868"
SQ SEQUENCE 505 AA; 53517 MW; FC17DB022C818D51 CRC64;
MAHHRGHRHI LLYVSLALSL GLALAEDATD PSDDTGSFDD VEAVSEEADL DPYSMSQELN
KRPNVDPYSY LPSVGKRAFD HYGFTGGLGK RKIDHFGFVG GLGKRQIDPL GFSGGIGKRY
DSFAYSAGLG KRGMDSLAFS GGLGKRGMDS LAFSGGLGKR GMDSLAFSGG LGKRGMDSLA
FSGGLGKRGM DSLAFSGGLG KRGMDSLAFS GGLGKRGMDS FTFAPGLGKR GMDSLAFAGG
LGKRMDGFAF APGLGKRMDS FAFAPGLGKR GMDSLAFAGG LGKRMDSFAF APGLGKRMDS
FAFAPGLGKR GLDRYGFVGG LGKRGMDHFA FTGGLGKRDS GEASGDLEEG KRGLDAYSFT
GALGKRGLDR YGFVGGLGKR GMDDFAFSPG LGKKRMDSFM FGSRLGKRGM DRFSFSGHLG
KRKMDQFSFG PGLGKRGFDH YGFTGGIGKR GFDHYGFTGG IGKRQLDPML FSGRLGKRSS
SEQEEEDVRQ VEKRSTTEEQ SSKSL
