ID   TRAU_ECOLI              Reviewed;         330 AA.
AC   P18471;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Protein TraU;
DE   Flags: Precursor;
GN   Name=traU; OrderedLocusNames=ECOK12F087;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2198250; DOI=10.1128/jb.172.8.4263-4270.1990;
RA   Moore D., Maneewannakul K., Maneewannakul S., Wu J.H., Ippen-Ihler K.,
RA   Bradley D.E.;
RT   "Characterization of the F-plasmid conjugative transfer gene traU.";
RL   J. Bacteriol. 172:4263-4270(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 325-330.
RC   STRAIN=K12;
RX   PubMed=2050638; DOI=10.1128/jb.173.12.3872-3878.1991;
RA   Maneewannakul S., Maneewannakul K., Ippen-Ihler K.;
RT   "Characterization of trbC, a new F plasmid tra operon gene that is
RT   essential to conjugative transfer.";
RL   J. Bacteriol. 173:3872-3878(1991).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13, AND SUBCELLULAR LOCATION.
RC   STRAIN=K12;
RX   PubMed=1355084; DOI=10.1128/jb.174.17.5567-5574.1992;
RA   Maneewannakul S., Maneewannakul K., Ippen-Ihler K.;
RT   "Characterization, localization, and sequence of F transfer region
RT   products: the pilus assembly gene product TraW and a new product, TrbI.";
RL   J. Bacteriol. 174:5567-5574(1992).
CC   -!- FUNCTION: Appears to be more essential to conjugal DNA transfer than to
CC       assembly of pilus filaments. Required for F plasmid conjugative
CC       transfer.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:1355084}.
CC   -!- MISCELLANEOUS: May be part of a periplasmic complex containing other
CC       proteins.
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DR   EMBL; M34695; AAA98088.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44198.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97957.1; -; Genomic_DNA.
DR   EMBL; M60427; AAA24913.1; -; Genomic_DNA.
DR   EMBL; M93106; AAA24690.1; -; Genomic_DNA.
DR   PIR; B37756; B37756.
DR   RefSeq; NP_061466.1; NC_002483.1.
DR   RefSeq; WP_000830185.1; NZ_CP014273.1.
DR   AlphaFoldDB; P18471; -.
DR   DIP; DIP-28102N; -.
DR   PRIDE; P18471; -.
DR   PATRIC; fig|83333.107.peg.625; -.
DR   PRO; PR:P18471; -.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   InterPro; IPR009649; TraU.
DR   Pfam; PF06834; TraU; 1.
PE   3: Inferred from homology;
KW   Conjugation; Periplasm; Plasmid; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..330
FT                   /note="Protein TraU"
FT                   /id="PRO_0000024509"
SQ   SEQUENCE   330 AA;  36787 MW;  8E2C3A4D45844A57 CRC64;
     MKRRLWLLML FLFAGHVPAA SADSACEGRF VNPITDICWS CIFPLSLGSI KVSQGKVPDT
     ANPSMPIQIC PAPPPLFRRI GLAIGYWEPM ALTDVTRSPG CMVNLGFSLP AFGKTAQGTA
     KKDEKQVNGA FYHVHWYKYP LTYWLNIITS LGCLEGGDMD IAYLSEIDPT WTDSSLTTIL
     NPEAVIFANP IAQGACAADA IASAFNMPLD VLFWCAGSQG SMYPFNGWVS NESSPLQSSL
     LVSERMAFKL HRQGMIMETI GKNNAVCNEY PSPILPKERW RYQMVNMYPD SGQCHPFGRS
     VTRWETGKNP PNTKKNFGYL MWRKRNCVFL