ID   TRAX1_ECOLI             Reviewed;         248 AA.
AC   P22709;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Protein TraX;
GN   Name=traX; OrderedLocusNames=ECOK12F105;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1916281; DOI=10.1016/0378-1119(91)90469-r;
RA   Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.;
RT   "Sequence and conservation of genes at the distal end of the transfer
RT   region on plasmids F and R6-5.";
RL   Gene 104:85-90(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-248.
RX   PubMed=2164585; DOI=10.1016/0022-2836(90)90145-c;
RA   Yoshioka Y., Fujita Y., Ohtsubo E.;
RT   "Nucleotide sequence of the promoter-distal region of the tra operon of
RT   plasmid R100, including traI (DNA helicase I) and traD genes.";
RL   J. Mol. Biol. 214:39-53(1990).
RN   [5]
RP   FUNCTION.
RX   PubMed=8444800; DOI=10.1128/jb.175.5.1375-1383.1993;
RA   Moore D., Hamilton C.M., Maneewannakul K., Mintz Y., Frost L.S.,
RA   Ippen-Ihler K.;
RT   "The Escherichia coli K-12 F plasmid gene traX is required for acetylation
RT   of F pilin.";
RL   J. Bacteriol. 175:1375-1383(1993).
CC   -!- FUNCTION: Responsible for the N-terminal acetylation of F pilin.
CC       {ECO:0000269|PubMed:8444800}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TraX family. {ECO:0000305}.
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DR   EMBL; M38047; AAA98091.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44201.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97975.1; -; Genomic_DNA.
DR   EMBL; X57429; CAA40675.1; -; Genomic_DNA.
DR   PIR; JQ1338; JQ1338.
DR   RefSeq; NP_061484.1; NC_002483.1.
DR   RefSeq; WP_000205776.1; NZ_CP014273.1.
DR   AlphaFoldDB; P22709; -.
DR   PRIDE; P22709; -.
DR   PATRIC; fig|83333.107.peg.606; -.
DR   PRO; PR:P22709; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008875; TraX.
DR   InterPro; IPR014125; TraX_Ftype.
DR   Pfam; PF05857; TraX; 1.
DR   TIGRFAMs; TIGR02755; TraX_Ftype; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..248
FT                   /note="Protein TraX"
FT                   /id="PRO_0000068478"
FT   TRANSMEM        39..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..248
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   248 AA;  27535 MW;  0F0E9DA4E3F48048 CRC64;
     MTTDNTNTTR NDSLVARTDT WLQSLLVWSP GQRDIIKTVA LVLMVLDHAN RILHLDQSWM
     FLAGRGAFPL FALVWGLNLS RHAHIRQLAI NRLWGWAVFS QFWYYFAGFP WYEGNILFAF
     AVAAQVLTWC ETRSGWRTAA AILMMALWGP LSGTSYGIAG LLMLAVSHRL YRAEDRMERL
     VLVACLLAVI PALNLATSDA AAVAGLVMTV LTVGLVSCAG KLLSRFWPGD FFPTFYACHL
     AVLGVLAL