TRAY1_ECOLI
ID TRAY1_ECOLI Reviewed; 131 AA.
AC P06627; P71247;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Relaxosome protein TraY;
GN Name=traY; OrderedLocusNames=ECOK12F073;
OS Escherichia coli (strain K12).
OG Plasmid F.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=F;
RX PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT "Analysis of the sequence and gene products of the transfer region of the F
RT sex factor.";
RL Microbiol. Rev. 58:162-210(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / CR63; PLASMID=F;
RA Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT "Complete nucleotide sequence of the F plasmid: its implications for
RT organization and diversification of plasmid genomes.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 13-131.
RC PLASMID=F;
RX PubMed=6368316; DOI=10.1016/0378-1119(83)90038-0;
RA Fowler T., Taylor L., Thompson R.;
RT "The control region of the F plasmid transfer operon: DNA sequence of the
RT traJ and traY genes and characterisation of the traY leads to Z promoter.";
RL Gene 26:79-89(1983).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-117.
RC STRAIN=ECOR 30, ECOR 37, ECOR 58, and ECOR 62; PLASMID=F;
RX PubMed=8807284; DOI=10.1093/genetics/143.3.1091;
RA Boyd E.F., Hill C.W., Rich S.M., Hartl D.L.;
RT "Mosaic structure of plasmids from natural populations of Escherichia
RT coli.";
RL Genetics 143:1091-1100(1996).
RN [5]
RP DNA-BINDING, FUNCTION IN DNA-BENDING, AND SUBUNIT.
RC PLASMID=F;
RX PubMed=8152370; DOI=10.1111/j.1365-2958.1994.tb00327.x;
RA Luo Y., Gao Q., Deonier R.C.;
RT "Mutational and physical analysis of F plasmid traY protein binding to
RT oriT.";
RL Mol. Microbiol. 11:459-469(1994).
RN [6]
RP FUNCTION IN F PLASMID NICKING.
RC PLASMID=F;
RX PubMed=7499339; DOI=10.1074/jbc.270.47.28381;
RA Nelson W.C., Howard M.T., Sherman J.A., Matson S.W.;
RT "The traY gene product and integration host factor stimulate Escherichia
RT coli DNA helicase I-catalyzed nicking at the F plasmid oriT.";
RL J. Biol. Chem. 270:28374-28380(1995).
RN [7]
RP CHARACTERIZATION OF RELAXOSOME ASSEMBLY ORDER.
RC PLASMID=F;
RX PubMed=7499340; DOI=10.1074/jbc.270.47.28374;
RA Howard M.T., Nelson W.C., Matson S.W.;
RT "Stepwise assembly of a relaxosome at the F plasmid origin of transfer.";
RL J. Biol. Chem. 270:28381-28386(1995).
RN [8]
RP FUNCTION IN GENE REGULATION.
RC PLASMID=F;
RX PubMed=8824627; DOI=10.1128/jb.178.19.5787-5789.1996;
RA Silverman P.M., Sholl A.;
RT "Effect of traY amber mutations on F-plasmid traY promoter activity in
RT vivo.";
RL J. Bacteriol. 178:5787-5789(1996).
RN [9]
RP FUNCTION IN TRAI NICKING, AND SUBUNIT.
RC PLASMID=F;
RX PubMed=17238924; DOI=10.1111/j.1365-2958.2006.05576.x;
RA Ragonese H., Haisch D., Villareal E., Choi J.H., Matson S.W.;
RT "The F plasmid-encoded TraM protein stimulates relaxosome-mediated cleavage
RT at oriT through an interaction with TraI.";
RL Mol. Microbiol. 63:1173-1184(2007).
CC -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC of ssDNA plasmid from a donor to a recipient cell. It is the central
CC mechanism by which antibiotic resistance and virulence factors are
CC propagated in bacterial populations. Part of the relaxosome, which
CC facilitates a site- and strand-specific cut in the origin of transfer
CC by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC and TraY to the oriT region, which then facilitates binding of TraI.
CC Upon binding to the oriT region TraY bends DNA 50 to 55 degrees. Also
CC positively regulates tra gene expression. {ECO:0000269|PubMed:17238924,
CC ECO:0000269|PubMed:7499339, ECO:0000269|PubMed:8152370,
CC ECO:0000269|PubMed:8824627}.
CC -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC transfer (oriT). Interacts with TraM, probably through its C-terminus.
CC {ECO:0000269|PubMed:17238924, ECO:0000269|PubMed:8152370}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
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DR EMBL; U01159; AAC44202.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97943.1; -; Genomic_DNA.
DR EMBL; K01147; AAA24909.1; -; Genomic_DNA.
DR EMBL; U50666; AAC44250.1; -; Genomic_DNA.
DR EMBL; U50662; AAC44246.1; -; Genomic_DNA.
DR EMBL; U50663; AAC44247.1; -; Genomic_DNA.
DR EMBL; U50664; AAC44248.1; -; Genomic_DNA.
DR PIR; B21874; BVECTY.
DR RefSeq; NP_061452.1; NC_002483.1.
DR RefSeq; NP_862918.1; NC_004998.1.
DR RefSeq; WP_001309237.1; NZ_SSZK01000064.1.
DR RefSeq; YP_009068352.1; NC_025139.1.
DR RefSeq; YP_009070617.1; NC_025175.1.
DR AlphaFoldDB; P06627; -.
DR PRIDE; P06627; -.
DR PRO; PR:P06627; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR008876; TraY.
DR Pfam; PF05509; TraY; 2.
PE 1: Evidence at protein level;
KW Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW Transcription regulation.
FT CHAIN 1..131
FT /note="Relaxosome protein TraY"
FT /id="PRO_0000068480"
FT VARIANT 63
FT /note="G -> D (in strain: ECOR 37)"
SQ SEQUENCE 131 AA; 15183 MW; D29A8654DE97F2AF CRC64;
MKRFGTRSAT GKMVKLKLPV DVESLLIEAS NRSGRSRSFE AVIRLKDHLH RYPKFNRAGN
IYGKSLVKYL TMRLDDETNQ LLIAAKNRSG WCKTDEAADR VIDHLIKFPD FYNSEIFREA
DKEEDITFNT L
