ID   TRAY2_ECOLX             Reviewed;          75 AA.
AC   P10512; P12251;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Relaxosome protein TraY;
GN   Name=traY;
OS   Escherichia coli.
OG   Plasmid IncFII R1-19 (R1 drd-19).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3009392; DOI=10.1128/jb.166.2.368-374.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Nucleotide sequences of the R1-19 plasmid transfer genes traM, finP, traJ,
RT   and traY and the traYZ promoter.";
RL   J. Bacteriol. 166:368-374(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2564189; DOI=10.1093/nar/17.4.1283;
RA   Koraimann G.M., Hoegenauer G.;
RT   "A stable core region of the tra operon mRNA of plasmid R1-19.";
RL   Nucleic Acids Res. 17:1283-1298(1989).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC       and TraY to the oriT region, which then facilitates binding of TraI.
CC       Also positively regulates tra gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC       TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC       transfer (oriT). Interacts with TraM, probably through its C-terminus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X13681; CAA31972.1; -; Genomic_DNA.
DR   EMBL; M19710; AAA92658.1; -; Genomic_DNA.
DR   PIR; C24544; C24544.
DR   RefSeq; WP_001254388.1; NZ_WWEV01000072.1.
DR   RefSeq; YP_003108301.1; NC_013122.1.
DR   RefSeq; YP_003829133.1; NC_014384.1.
DR   RefSeq; YP_003829258.1; NC_014385.1.
DR   AlphaFoldDB; P10512; -.
DR   SMR; P10512; -.
DR   OMA; QRYPDFY; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR008876; TraY.
DR   Pfam; PF05509; TraY; 1.
PE   3: Inferred from homology;
KW   Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..75
FT                   /note="Relaxosome protein TraY"
FT                   /id="PRO_0000068481"
SQ   SEQUENCE   75 AA;  9072 MW;  F89EFA1DF714CCDB CRC64;
     MRRRNARGGI SRTVSVYLDE DTNNRLIRAK DRSGRSKTIE VQIRLRDHLK RFPDFYNEEI
     FREVIEENES TFKEL