ID   TRAY3_ECOLX             Reviewed;          75 AA.
AC   P05835;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Relaxosome protein TraY;
GN   Name=traY;
OS   Escherichia coli.
OG   Plasmid IncFII R100-1, and Plasmid IncFII R100 (NR1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFII R100-1;
RX   PubMed=3531163; DOI=10.1128/jb.168.1.132-139.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Origin of transfer of IncF plasmids and nucleotide sequences of the type
RT   II oriT, traM, and traY alleles from ColB4-K98 and the type IV traY allele
RT   from R100-1.";
RL   J. Bacteriol. 168:132-139(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=IncFII R100 (NR1);
RX   PubMed=2836369; DOI=10.1128/jb.170.6.2749-2757.1988;
RA   Inamoto S., Yoshioka Y., Ohtsubo E.;
RT   "Identification and characterization of the products from the traJ and traY
RT   genes of plasmid R100.";
RL   J. Bacteriol. 170:2749-2757(1988).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC       and TraY to the oriT region, which then facilitates binding of TraI.
CC       Also positively regulates tra gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC       TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC       transfer (oriT). Interacts with TraM, probably through its C-terminus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
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DR   EMBL; M15136; AAA26076.1; -; Genomic_DNA.
DR   EMBL; M20941; AAA26073.1; -; Genomic_DNA.
DR   PIR; C25033; BVECRY.
DR   RefSeq; NP_957599.1; NC_005327.1.
DR   RefSeq; WP_000089263.1; NZ_WVVN01000094.1.
DR   RefSeq; YP_001096467.1; NC_009133.1.
DR   RefSeq; YP_003108229.1; NC_013121.1.
DR   RefSeq; YP_006952237.1; NC_019057.1.
DR   RefSeq; YP_006953321.1; NC_019071.1.
DR   RefSeq; YP_006953420.1; NC_019072.1.
DR   RefSeq; YP_006953947.1; NC_019090.1.
DR   RefSeq; YP_006954268.1; NC_019095.1.
DR   RefSeq; YP_006990759.1; NC_019424.1.
DR   RefSeq; YP_007447545.1; NC_020278.2.
DR   RefSeq; YP_009070966.1; NC_025177.1.
DR   RefSeq; YP_788061.1; NC_008460.1.
DR   AlphaFoldDB; P05835; -.
DR   SMR; P05835; -.
DR   GeneID; 58462407; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR008876; TraY.
DR   Pfam; PF05509; TraY; 1.
PE   3: Inferred from homology;
KW   Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..75
FT                   /note="Relaxosome protein TraY"
FT                   /id="PRO_0000068482"
SQ   SEQUENCE   75 AA;  8542 MW;  88D4B04C4B5DE07A CRC64;
     MSRNIIRPAP GNKVLLVLDD ATNHKLLGAR ERSGRTKTNE VLVRLRDHLN RFPDFYNLDA
     IKEGAEETDS IIKDL