ID   TRAY4_ECOLX             Reviewed;          75 AA.
AC   P05836;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Relaxosome protein TraY;
GN   Name=traY;
OS   Escherichia coli.
OG   Plasmid ColB4-K98.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3531163; DOI=10.1128/jb.168.1.132-139.1986;
RA   Finlay B.B., Frost L.S., Paranchych W.;
RT   "Origin of transfer of IncF plasmids and nucleotide sequences of the type
RT   II oriT, traM, and traY alleles from ColB4-K98 and the type IV traY allele
RT   from R100-1.";
RL   J. Bacteriol. 168:132-139(1986).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC       and TraY to the oriT region, which then facilitates binding of TraI.
CC       Also positively regulates tra gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC       TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC       transfer (oriT). Interacts with TraM, probably through its C-terminus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
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DR   EMBL; M15135; AAB04665.1; -; Genomic_DNA.
DR   PIR; B25033; BVECKY.
DR   RefSeq; WP_001254386.1; NZ_WVVZ01000042.1.
DR   RefSeq; YP_006953836.1; NC_019089.1.
DR   RefSeq; YP_009066477.1; NC_025106.1.
DR   AlphaFoldDB; P05836; -.
DR   SMR; P05836; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR008876; TraY.
DR   Pfam; PF05509; TraY; 1.
PE   3: Inferred from homology;
KW   Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..75
FT                   /note="Relaxosome protein TraY"
FT                   /id="PRO_0000068483"
SQ   SEQUENCE   75 AA;  9005 MW;  DC2828C4F714CCD4 CRC64;
     MRRRNARGGI SRTVSVYLDE DTNNRLIKAK DRSGRSKTIE VQIRLRDHLK RFPDFYNEEI
     FREVTEESES TFKEL