ID   TRAY5_ECOLX             Reviewed;          71 AA.
AC   Q00736;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Relaxosome protein TraY;
GN   Name=traY;
OS   Escherichia coli.
OG   Plasmid IncFI P307.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=711;
RX   PubMed=2096398; DOI=10.1016/0147-619x(90)90014-4;
RA   Graus-Goeldner A., Graus H., Schlacher T., Hoegenauer G.;
RT   "The sequences of genes bordering oriT in the enterotoxin plasmid P307:
RT   comparison with the sequences of plasmids F and R1.";
RL   Plasmid 24:119-131(1990).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC       and TraY to the oriT region, which then facilitates binding of TraI.
CC       Also positively regulates tra gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC       TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC       transfer (oriT). Interacts with TraM, probably through its C-terminus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
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DR   EMBL; M62986; AAA25524.1; -; Genomic_DNA.
DR   PIR; D37390; D37390.
DR   RefSeq; WP_071782156.1; NZ_QZWA01000502.1.
DR   AlphaFoldDB; Q00736; -.
DR   SMR; Q00736; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR008876; TraY.
DR   Pfam; PF05509; TraY; 1.
PE   3: Inferred from homology;
KW   Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..71
FT                   /note="Relaxosome protein TraY"
FT                   /id="PRO_0000068484"
SQ   SEQUENCE   71 AA;  8103 MW;  9A031E0ECBE99263 CRC64;
     MSRGRTRAAP GNKVLLILDE TTNQKLLAAR DRSGRTKTNE VFIRLKDHLN RFPDFYNSSL
     VKEEAEGIDD I