ID   TRAY8_ECOLX             Reviewed;          20 AA.
AC   P33789;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 3.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Relaxosome protein TraY;
DE   Flags: Fragment;
GN   Name=traY;
OS   Escherichia coli.
OG   Plasmid IncFV pED208.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1943709; DOI=10.1111/j.1365-2958.1991.tb01927.x;
RA   di Laurenzio L., Frost L.S., Finlay B.B., Paranchych W.;
RT   "Characterization of the oriT region of the IncFV plasmid pED208.";
RL   Mol. Microbiol. 5:1779-1790(1991).
CC   -!- FUNCTION: Conjugative DNA transfer (CDT) is the unidirectional transfer
CC       of ssDNA plasmid from a donor to a recipient cell. It is the central
CC       mechanism by which antibiotic resistance and virulence factors are
CC       propagated in bacterial populations. Part of the relaxosome, which
CC       facilitates a site- and strand-specific cut in the origin of transfer
CC       by TraI, at the nic site. Relaxosome formation requires binding of IHF
CC       and TraY to the oriT region, which then facilitates binding of TraI.
CC       Also positively regulates tra gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Part of the relaxosome, a complex composed of plasmid encoded
CC       TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of
CC       transfer (oriT). Interacts with TraM, probably through its C-terminus
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TraY family. {ECO:0000305}.
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DR   EMBL; X59611; CAA42176.1; -; Genomic_DNA.
DR   PIR; S16478; S16478.
DR   AlphaFoldDB; P33789; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Activator; Conjugation; Cytoplasm; DNA-binding; Plasmid; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..>20
FT                   /note="Relaxosome protein TraY"
FT                   /id="PRO_0000068485"
FT   NON_TER         20
SQ   SEQUENCE   20 AA;  2239 MW;  1B7044134939C0DD CRC64;
     MKEPKSNIRK LIDIGGLIGR