ID   TRA_STRLI               Reviewed;         621 AA.
AC   P22409;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Protein Tra;
GN   Name=tra; Synonyms=kilA;
OS   Streptomyces lividans.
OG   Plasmid pIJ101.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1916;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3170481; DOI=10.1128/jb.170.10.4634-4651.1988;
RA   Kendall K.J., Cohen S.N.;
RT   "Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101
RT   and correlation of the sequence with genetic properties.";
RL   J. Bacteriol. 170:4634-4651(1988).
CC   -!- FUNCTION: Major protein required for plasmid transfer.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; M21778; AAA88410.1; -; Genomic_DNA.
DR   PIR; G31844; G31844.
DR   RefSeq; NP_040447.1; NC_001387.1.
DR   RefSeq; WP_010889919.1; NC_001387.1.
DR   AlphaFoldDB; P22409; -.
DR   TCDB; 3.A.12.2.1; the septal dna translocator (s-dna-t) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR002543; FtsK_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01580; FtsK_SpoIIIE; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50901; FTSK; 1.
PE   4: Predicted;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..621
FT                   /note="Protein Tra"
FT                   /id="PRO_0000098326"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        564..584
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          275..465
FT                   /note="FtsK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT   BINDING         290..297
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
SQ   SEQUENCE   621 AA;  66420 MW;  BD5EF04AA98E7430 CRC64;
     MAPTLAPFAA RWDAEADRRM ALRTPEHLKA LMDAQKGYNS ARSTAATAKS QRTAARAASK
     NPLSTVRRAA RTADKAARTH RDQAKTKLKA ARKDYPATLR ARAVQAHAMH AVPGAGISAL
     GWDQAGAWPV AGSLALIAAN VAALVIGRRK VAVAVADELS AEERRLVERL DPSYWAQHAD
     ERGLVGTVTT PVQVTPAGLV TTVRLDGRWK PSAFKAKHEE IRALLGARTD LRMEIKAGSH
     GDRAVITLRT RSAADGIDLT GWTPGAPWGV DTVTGEPVQV PLGRRMLIAG TSGSGKSWST
     RALLAEGSEY ADHRLVVVDP KRVEAINWQH RARTAISIED VLDVTDELVE EMHERLELIP
     RGQDVIQISP ERPRITVFID EGAEVIAMAK KTRAKGSKEE PGDPDWSRIM ENLSTLARMA
     RAAEIILIWA TQKPTMDAKG GIDPQISAQI TYRAALALST SGESRVVFGE DATEKGWHAH
     ELPMPGVAML RSGPKVQPHP INTRAFSPAD VIALPDRPVW RRQESPARSA GASAPAPLRL
     VKETAPAAEV PAQPTKAPTN REKVAAAIGT GATTVADVAT VTGINKGSVS KAVKQLLDAG
     EVLRSEDGSL SVVTQVGEVS A