ID   TRBI_ECOLI              Reviewed;         128 AA.
AC   P18006;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Protein TrbI;
GN   Name=trbI; OrderedLocusNames=ECOK12F085;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=K12;
RX   PubMed=1355084; DOI=10.1128/jb.174.17.5567-5574.1992;
RA   Maneewannakul S., Maneewannakul K., Ippen-Ihler K.;
RT   "Characterization, localization, and sequence of F transfer region
RT   products: the pilus assembly gene product TraW and a new product, TrbI.";
RL   J. Bacteriol. 174:5567-5574(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7915817; DOI=10.1128/mr.58.2.162-210.1994;
RA   Frost L.S., Ippen-Ihler K., Skurray R.A.;
RT   "Analysis of the sequence and gene products of the transfer region of the F
RT   sex factor.";
RL   Microbiol. Rev. 58:162-210(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RC   STRAIN=K12;
RX   PubMed=2265751; DOI=10.1016/0378-1119(90)90354-t;
RA   Schandel K.A., Maneewannakul S., Vonder Haar R.A., Ippen-Ihler K.,
RA   Webster R.E.;
RT   "Nucleotide sequence of the F plasmid gene, traC, and identification of its
RT   product.";
RL   Gene 96:137-140(1990).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / X90; PLASMID=F;
RX   PubMed=24889811; DOI=10.1111/mmi.12658;
RA   Beck C.M., Diner E.J., Kim J.J., Low D.A., Hayes C.S.;
RT   "The F pilus mediates a novel pathway of CDI toxin import.";
RL   Mol. Microbiol. 93:276-290(2014).
CC   -!- FUNCTION: May influence the kinetics of pilus outgrowth and/or
CC       retraction (PubMed:1355084). Overexpression phenocopies the deletion
CC       mutation; i.e. causes resistance to F-pilus-specific phage and CdiA-CT
CC       toxin (PubMed:24889811). {ECO:0000269|PubMed:24889811,
CC       ECO:0000305|PubMed:1355084}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:1355084};
CC       Single-pass membrane protein {ECO:0000305|PubMed:1355084}.
CC   -!- DISRUPTION PHENOTYPE: Expresses approximately normal numbers of F pili
CC       that are unusually long, remains plasmid transfer proficient, displays
CC       increased resistance to F-pilus-specific phage (PubMed:1355084).
CC       Resistant to phage R17 and M13, conjugates at lower efficiency (about
CC       10% of wild-type), resistant to CdiA-CT toxin (PubMed:24889811).
CC       {ECO:0000269|PubMed:1355084, ECO:0000269|PubMed:24889811}.
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DR   EMBL; M93106; AAA24688.1; -; Genomic_DNA.
DR   EMBL; U01159; AAC44211.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97955.1; -; Genomic_DNA.
DR   EMBL; M30936; AAA24906.1; -; Genomic_DNA.
DR   PIR; A42938; A42938.
DR   RefSeq; NP_061464.1; NC_002483.1.
DR   AlphaFoldDB; P18006; -.
DR   DIP; DIP-28101N; -.
DR   PRIDE; P18006; -.
DR   PRO; PR:P18006; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR014115; TrbI_Ftype.
DR   Pfam; PF09677; TrbI_Ftype; 1.
DR   TIGRFAMs; TIGR02744; TrbI_Ftype; 1.
PE   4: Predicted;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..128
FT                   /note="Protein TrbI"
FT                   /id="PRO_0000068493"
FT   TRANSMEM        18..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        113
FT                   /note="S -> T (in Ref. 3; BAA97955)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   128 AA;  14132 MW;  03DAAAC65397A703 CRC64;
     MSSTQKPADV TAERRSHWWW TVPGCLAMVL LNAAVSYGIV RLNAPVTVAF NMKQTVDAFF
     DSASQKQLSE AQSKALSARF NTALEASLQA WQQKHHAVIL VSPAVVQGAP DISREIQQDI
     ARRMRAEP