ID   TRBP2_DANRE             Reviewed;         346 AA.
AC   Q7SXR1;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=RISC-loading complex subunit tarbp2 {ECO:0000255|HAMAP-Rule:MF_03034};
GN   Name=tarbp2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for formation of the RNA induced silencing complex
CC       (RISC). Component of the RISC loading complex (RLC), also known as the
CC       micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1,
CC       ago2 and tarbp2. Within the RLC/miRLC, dicer1 and tarbp2 are required
CC       to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load
CC       them onto ago2. ago2 bound to the mature miRNA constitutes the minimal
CC       RISC and may subsequently dissociate from dicer1 and tarbp2. May also
CC       play a role in the production of short interfering RNAs (siRNAs) from
CC       double-stranded RNA (dsRNA) by dicer1. {ECO:0000255|HAMAP-
CC       Rule:MF_03034}.
CC   -!- SUBUNIT: Self-associates. Component of the RISC loading complex (RLC),
CC       or micro-RNA (miRNA) loading complex (miRLC), which is composed of
CC       dicer1, ago2 and tarbp2. Note that the trimeric RLC/miRLC is also
CC       referred to as RISC. {ECO:0000255|HAMAP-Rule:MF_03034}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03034}.
CC   -!- SIMILARITY: Belongs to the TARBP2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03034}.
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DR   EMBL; BC055390; AAH55390.1; -; mRNA.
DR   RefSeq; NP_956291.1; NM_199997.1.
DR   AlphaFoldDB; Q7SXR1; -.
DR   SMR; Q7SXR1; -.
DR   STRING; 7955.ENSDARP00000094231; -.
DR   PaxDb; Q7SXR1; -.
DR   GeneID; 336141; -.
DR   KEGG; dre:336141; -.
DR   CTD; 6895; -.
DR   ZFIN; ZDB-GENE-030131-8085; tarbp2.
DR   eggNOG; KOG3732; Eukaryota.
DR   InParanoid; Q7SXR1; -.
DR   OrthoDB; 1093169at2759; -.
DR   PhylomeDB; Q7SXR1; -.
DR   Reactome; R-DRE-203927; MicroRNA (miRNA) biogenesis.
DR   Reactome; R-DRE-426486; Small interfering RNA (siRNA) biogenesis.
DR   PRO; PR:Q7SXR1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0016442; C:RISC complex; ISS:UniProtKB.
DR   GO; GO:0070578; C:RISC-loading complex; ISS:UniProtKB.
DR   GO; GO:0003725; F:double-stranded RNA binding; IBA:GO_Central.
DR   GO; GO:0035198; F:miRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070883; F:pre-miRNA binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035197; F:siRNA binding; IBA:GO_Central.
DR   GO; GO:0031054; P:pre-miRNA processing; IEA:UniProtKB-UniRule.
DR   GO; GO:1903798; P:regulation of miRNA maturation; IEA:InterPro.
DR   GO; GO:0070920; P:regulation of production of small RNA involved in gene silencing by RNA; IBA:GO_Central.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0046782; P:regulation of viral transcription; IEA:InterPro.
DR   GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR   CDD; cd10844; DSRM_TARBP2_rpt2; 1.
DR   CDD; cd19893; DSRM_TARBP2_rpt3; 1.
DR   HAMAP; MF_03034; TRBP2; 1.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR032478; Staufen_C.
DR   InterPro; IPR028605; TRBP2.
DR   InterPro; IPR044470; TRBP2_DSRM_2.
DR   InterPro; IPR044471; TRBP2_DSRM_3.
DR   PANTHER; PTHR46205:SF1; PTHR46205:SF1; 1.
DR   Pfam; PF00035; dsrm; 2.
DR   Pfam; PF16482; Staufen_C; 1.
DR   SMART; SM00358; DSRM; 3.
DR   PROSITE; PS50137; DS_RBD; 3.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing; Translation regulation.
FT   CHAIN           1..346
FT                   /note="RISC-loading complex subunit tarbp2"
FT                   /id="PRO_0000373972"
FT   DOMAIN          30..97
FT                   /note="DRBM 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
FT   DOMAIN          139..207
FT                   /note="DRBM 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
FT   DOMAIN          273..341
FT                   /note="DRBM 3"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
SQ   SEQUENCE   346 AA;  37435 MW;  6B8706EE2975FBE8 CRC64;
     MNDEKSQDNG KRNSGYTSIE QMLAVNPGKT PISLLQEYGT RIGKTPVYDL LKAEGQAHQP
     NFTFRVSVGD INCTGHGPSK KAAKHKAAEA ALKMLKGGML GGIGGNGMEG DGFVGIEMEG
     ECPQSEMKSS SSTQQAECNP VGALQELVVQ KGWRLPEYTV TQESGPAHRK EFTMTCRVER
     FVEIGSGTSK KLAKRNAAAK MLSRIHDVPV DMRSSHEAEA EDDTFNMQIG GRLEGGKSKG
     LGCTWDSLRN SAGEKILQLR CHPLGQSDSI DSNFCSLLRE LSEEQRFGVS YLDIEERSLS
     GLYQCLVELS TQPITVCHGF ASSLDAARAS AAHNALQYLK IMAGGK