TRBP2_XENTR
ID TRBP2_XENTR Reviewed; 351 AA.
AC Q5BJ52;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=RISC-loading complex subunit tarbp2 {ECO:0000255|HAMAP-Rule:MF_03034};
GN Name=tarbp2;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for formation of the RNA induced silencing complex
CC (RISC). Component of the RISC loading complex (RLC), also known as the
CC micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1,
CC ago2 and tarbp2. Within the RLC/miRLC, dicer1 and tarbp2 are required
CC to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load
CC them onto ago2. ago2 bound to the mature miRNA constitutes the minimal
CC RISC and may subsequently dissociate from dicer1 and tarbp2. May also
CC play a role in the production of short interfering RNAs (siRNAs) from
CC double-stranded RNA (dsRNA) by dicer1. {ECO:0000255|HAMAP-
CC Rule:MF_03034}.
CC -!- SUBUNIT: Self-associates. Component of the RISC loading complex (RLC),
CC or micro-RNA (miRNA) loading complex (miRLC), which is composed of
CC dicer1, ago2 and tarbp2. Note that the trimeric RLC/miRLC is also
CC referred to as RISC. {ECO:0000255|HAMAP-Rule:MF_03034}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03034}.
CC -!- SIMILARITY: Belongs to the TARBP2 family. {ECO:0000255|HAMAP-
CC Rule:MF_03034}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH91619.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC091619; AAH91619.1; ALT_INIT; mRNA.
DR RefSeq; NP_001025646.1; NM_001030475.1.
DR RefSeq; XP_012812552.1; XM_012957098.2.
DR AlphaFoldDB; Q5BJ52; -.
DR SMR; Q5BJ52; -.
DR STRING; 8364.ENSXETP00000060479; -.
DR DNASU; 595034; -.
DR GeneID; 595034; -.
DR KEGG; xtr:595034; -.
DR CTD; 6895; -.
DR Xenbase; XB-GENE-491800; tarbp2.
DR eggNOG; KOG3732; Eukaryota.
DR InParanoid; Q5BJ52; -.
DR OrthoDB; 1093169at2759; -.
DR Reactome; R-XTR-203927; MicroRNA (miRNA) biogenesis.
DR Reactome; R-XTR-426486; Small interfering RNA (siRNA) biogenesis.
DR Proteomes; UP000008143; Chromosome 2.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0016442; C:RISC complex; ISS:UniProtKB.
DR GO; GO:0070578; C:RISC-loading complex; ISS:UniProtKB.
DR GO; GO:0003725; F:double-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0035198; F:miRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070883; F:pre-miRNA binding; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035197; F:siRNA binding; IBA:GO_Central.
DR GO; GO:0031054; P:pre-miRNA processing; IEA:UniProtKB-UniRule.
DR GO; GO:1903798; P:regulation of miRNA maturation; IEA:InterPro.
DR GO; GO:0070920; P:regulation of production of small RNA involved in gene silencing by RNA; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0046782; P:regulation of viral transcription; IEA:InterPro.
DR GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR CDD; cd19890; DSRM_TARBP2_rpt1; 1.
DR CDD; cd10844; DSRM_TARBP2_rpt2; 1.
DR CDD; cd19893; DSRM_TARBP2_rpt3; 1.
DR HAMAP; MF_03034; TRBP2; 1.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR032478; Staufen_C.
DR InterPro; IPR028605; TRBP2.
DR InterPro; IPR044469; TRBP2_DSRM_1.
DR InterPro; IPR044470; TRBP2_DSRM_2.
DR InterPro; IPR044471; TRBP2_DSRM_3.
DR PANTHER; PTHR46205:SF1; PTHR46205:SF1; 1.
DR Pfam; PF00035; dsrm; 2.
DR Pfam; PF16482; Staufen_C; 1.
DR SMART; SM00358; DSRM; 3.
DR PROSITE; PS50137; DS_RBD; 3.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Repeat; RNA-binding;
KW RNA-mediated gene silencing; Translation regulation.
FT CHAIN 1..351
FT /note="RISC-loading complex subunit tarbp2"
FT /id="PRO_0000373975"
FT DOMAIN 29..96
FT /note="DRBM 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
FT DOMAIN 150..218
FT /note="DRBM 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
FT DOMAIN 278..346
FT /note="DRBM 3"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03034"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..240
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 351 AA; 38227 MW; F004AF492E666A35 CRC64;
MSENGDCENQ TSSGFPSIEQ MLASSPGKTP ISLLQEYGTR VGKTPVYDLL KAEGQAHQPN
FTFRVSVGDI NCTGQGPSKK AAKHKAAEVA LSLLKGGDMF GMMCEENSVM LSVEQPVELR
EVADVSPPPT NRNHTIEMKP PLSAQQSECN PVGALQELVV QKGWRLPEYT VTQESGPAHR
KEFTMTCRVE RFLEIGSGTS KKLAKRNAAA KMLLQIHRVP AEHRESGETE PEEDQFSMGK
LDGSRGRGTA CTWDSLRNSS GEKILHLRSN PLTILSSGFC SLLQDLSEEQ SFQISYLDID
EPSLSGLYQC LVELSTQPTT VCHGSATTRD AARANAAHNA LQYLKIMAGG K
