TRCR_MYCTU
ID TRCR_MYCTU Reviewed; 257 AA.
AC L7N689; I6Y9F4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2013, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Transcriptional regulatory protein TrcR {ECO:0000305};
DE AltName: Full=Tuberculosis regulatory component response regulator {ECO:0000303|PubMed:10089160};
GN Name=trcR {ECO:0000303|PubMed:10089160};
GN OrderedLocusNames=Rv1033c {ECO:0000312|EMBL:CCP43784.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, AND PHOSPHORYLATION.
RX PubMed=10089160; DOI=10.1006/mpat.1998.0265;
RA Haydel S.E., Dunlap N.E., Benjamin W.H. Jr.;
RT "In vitro evidence of two-component system phosphorylation between the
RT Mycobacterium tuberculosis TrcR/TrcS proteins.";
RL Microb. Pathog. 26:195-206(1999).
RN [3]
RP FUNCTION, DNA-BINDING, AND INDUCTION.
RX PubMed=11914351; DOI=10.1128/jb.184.8.2192-2203.2002;
RA Haydel S.E., Benjamin W.H. Jr., Dunlap N.E., Clark-Curtiss J.E.;
RT "Expression, autoregulation, and DNA binding properties of the
RT Mycobacterium tuberculosis TrcR response regulator.";
RL J. Bacteriol. 184:2192-2203(2002).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=16352831; DOI=10.1128/jb.188.1.150-159.2006;
RA Haydel S.E., Clark-Curtiss J.E.;
RT "The Mycobacterium tuberculosis TrcR response regulator represses
RT transcription of the intracellularly expressed Rv1057 gene, encoding a
RT seven-bladed beta-propeller.";
RL J. Bacteriol. 188:150-159(2006).
RN [5]
RP FUNCTION.
RX PubMed=22099420; DOI=10.1016/j.tube.2011.10.024;
RA Pang X., Cao G., Neuenschwander P.F., Haydel S.E., Hou G., Howard S.T.;
RT "The beta-propeller gene Rv1057 of Mycobacterium tuberculosis has a complex
RT promoter directly regulated by both the MprAB and TrcRS two-component
RT systems.";
RL Tuberculosis 91:S142-S149(2011).
CC -!- FUNCTION: Member of the two-component regulatory system TrcS/TrcR
CC (PubMed:10089160, PubMed:11914351). Activates its own expression by
CC binding specifically to the AT-rich sequence of the trcR promoter
CC region (PubMed:11914351). Also negatively regulates the expression of
CC Rv1057 by binding to an AT-rich sequences within the Rv1057 upstream
CC sequence (PubMed:16352831, PubMed:22099420). The TrcR-TrcS regulatory
CC system may act as a transition regulatory system involved in adapting
CC to an intracellular environment and transitioning from latency to
CC reactivation (PubMed:11914351). {ECO:0000269|PubMed:10089160,
CC ECO:0000269|PubMed:11914351, ECO:0000269|PubMed:16352831,
CC ECO:0000269|PubMed:22099420}.
CC -!- INDUCTION: Expressed in broth-grown cultures and after 18 hours of
CC M.tuberculosis growth in cultured human primary macrophages, but not
CC after longer periods of macrophage infection (PubMed:11914351).
CC Positively autoregulated (PubMed:11914351).
CC {ECO:0000269|PubMed:11914351}.
CC -!- PTM: Phosphorylated by TrcS. {ECO:0000269|PubMed:10089160}.
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DR EMBL; AL123456; CCP43784.1; -; Genomic_DNA.
DR RefSeq; NP_215549.1; NC_000962.3.
DR RefSeq; WP_003405328.1; NZ_NVQJ01000018.1.
DR AlphaFoldDB; L7N689; -.
DR SMR; L7N689; -.
DR STRING; 83332.Rv1033c; -.
DR PaxDb; L7N689; -.
DR PRIDE; L7N689; -.
DR DNASU; 887957; -.
DR GeneID; 45425007; -.
DR GeneID; 887957; -.
DR KEGG; mtu:Rv1033c; -.
DR PATRIC; fig|83332.111.peg.1147; -.
DR TubercuList; Rv1033c; -.
DR eggNOG; COG0745; Bacteria.
DR OMA; YIQTHIG; -.
DR PhylomeDB; L7N689; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..257
FT /note="Transcriptional regulatory protein TrcR"
FT /id="PRO_0000451063"
FT DOMAIN 33..147
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 158..255
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 82
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 257 AA; 29209 MW; 164B4DF50FDA80A9 CRC64;
MTTMSGYTRS QRPRQAILGQ LPRIHRADGS PIRVLLVDDE PALTNLVKMA LHYEGWDVEV
AHDGQEAIAK FDKVGPDVLV LDIMLPDVDG LEILRRVRES DVYTPTLFLT ARDSVMDRVT
GLTSGADDYM TKPFSLEELV ARLRGLLRRS SHLERPADEA LRVGDLTLDG ASREVTRDGT
PISLSSTEFE LLRFLMRNPR RALSRTEILD RVWNYDFAGR TSIVDLYISY LRKKIDSDRE
PMIHTVRGIG YMLRPPE
