BUD23_BOVIN
ID BUD23_BOVIN Reviewed; 281 AA.
AC Q58DP0; Q2TBN2;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Probable 18S rRNA (guanine-N(7))-methyltransferase {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:O43709};
DE AltName: Full=Bud site selection protein 23 homolog;
DE AltName: Full=Williams-Beuren syndrome chromosomal region 22 protein homolog;
DE AltName: Full=rRNA methyltransferase and ribosome maturation factor {ECO:0000250|UniProtKB:O43709};
GN Name=BUD23 {ECO:0000250|UniProtKB:O43709}; Synonyms=WBSCR22;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC specifically methylates the N(7) position of a guanine in 18S rRNA.
CC Requires the methyltransferase adapter protein TRM112 for full rRNA
CC methyltransferase activity. Involved in the pre-rRNA processing steps
CC leading to small-subunit rRNA production independently of its RNA-
CC modifying catalytic activity. Important for biogenesis end export of
CC the 40S ribosomal subunit independent on its methyltransferase
CC activity. Locus-specific steroid receptor coactivator. Potentiates
CC transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2),
CC androgen (AR) and progesterone (PGR) receptors. Required for the
CC maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate
CC NR3C1 loading on the response elements. Required for maintenance of
CC dimethylation on histone H3 'Lys-79' (H3K79me2), although direct
CC histone methyltransferase activity is not observed in vitro.
CC {ECO:0000250, ECO:0000250|UniProtKB:O43709}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a guanosine in 18S rRNA + S-adenosyl-L-methionine = an N(7)-
CC methylguanosine in 18S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:54584, Rhea:RHEA-COMP:13937, Rhea:RHEA-COMP:13938,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; Evidence={ECO:0000250|UniProtKB:O43709};
CC -!- SUBUNIT: Heterodimer with TRMT112; this heterodimerization is necessary
CC for the metabolic stability and activity of the catalytic subunit
CC BUD23. Interacts with GRIP1. {ECO:0000250|UniProtKB:O43709}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43709}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:O43709}. Cytoplasm, perinuclear
CC region {ECO:0000250|UniProtKB:O43709}. Cytoplasm
CC {ECO:0000250|UniProtKB:O43709}. Note=Localized diffusely throughout the
CC nucleus and the cytoplasm. Localizes to a polarized perinuclear
CC structure, overlapping partially with the Golgi and lysosomes.
CC Localization is not affected by glucocorticoid treatment.
CC {ECO:0000250|UniProtKB:O43709}.
CC -!- PTM: May be ubiquitinated and targeted to degradation in response to
CC pro-inflammatory cytokine signaling. {ECO:0000250|UniProtKB:O43709}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. BUD23/WBSCR22 family. {ECO:0000305}.
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DR EMBL; BT021557; AAX46404.1; -; mRNA.
DR EMBL; BC109889; AAI09890.1; -; mRNA.
DR RefSeq; NP_001029629.2; NM_001034457.2.
DR AlphaFoldDB; Q58DP0; -.
DR SMR; Q58DP0; -.
DR STRING; 9913.ENSBTAP00000022697; -.
DR PaxDb; Q58DP0; -.
DR PRIDE; Q58DP0; -.
DR GeneID; 513878; -.
DR KEGG; bta:513878; -.
DR CTD; 114049; -.
DR eggNOG; KOG1541; Eukaryota.
DR HOGENOM; CLU_055194_0_2_1; -.
DR InParanoid; Q58DP0; -.
DR OrthoDB; 1138059at2759; -.
DR TreeFam; TF300750; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0016435; F:rRNA (guanine) methyltransferase activity; ISS:UniProtKB.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:2000234; P:positive regulation of rRNA processing; ISS:UniProtKB.
DR GO; GO:0070476; P:rRNA (guanine-N7)-methylation; ISS:UniProtKB.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR039769; Bud23-like.
DR InterPro; IPR022238; Bud23_C.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12734; PTHR12734; 1.
DR Pfam; PF08241; Methyltransf_11; 1.
DR Pfam; PF12589; WBS_methylT; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 2: Evidence at transcript level;
KW Chromatin regulator; Cytoplasm; Methyltransferase; Nucleus;
KW Reference proteome; Ribosome biogenesis; rRNA processing;
KW S-adenosyl-L-methionine; Transcription; Transcription regulation;
KW Transferase; Ubl conjugation.
FT CHAIN 1..281
FT /note="Probable 18S rRNA (guanine-N(7))-methyltransferase"
FT /id="PRO_0000247014"
FT REGION 256..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 3
FT /note="F -> I (in Ref. 2; AAI09890)"
FT /evidence="ECO:0000305"
FT CONFLICT 85
FT /note="P -> S (in Ref. 2; AAI09890)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 281 AA; 31687 MW; F45ACE99A6B46D9D CRC64;
MAFRGRRPEL RGPPELYYDK NEARKYVRNS RMIDVQIKMT GRALELLCVP EDKPCYVLDI
GCGTGLSGDY LSDEGHYWVG IDISPAMLDE ALDRETQGDV ILGDMGQGIP FKPGTFDACI
SISAVQWLCN ANKKSDIPAK RLYCFFSSLY SVLVRGGRAV LQLYPENSEQ LELITTQATR
AGFTGGVVVD YPNSAKAKKF YLCLFSGPST SLPEGLSEDT EEEKPAESTF TADRIPYRIA
RRGVVRKSRE WVLEKKARRR RQGKEVCPDT QYTGRKRKPR F
