BUD23_DICDI
ID BUD23_DICDI Reviewed; 287 AA.
AC Q55DA6;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Probable 18S rRNA (guanine-N(7))-methyltransferase {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:O43709};
DE AltName: Full=Bud site selection protein 23 homolog;
DE AltName: Full=Williams-Beuren syndrome chromosomal region 22 protein homolog;
GN ORFNames=DDB_G0269722;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC specifically methylates the N(7) position of a guanine in 18S rRNA.
CC Important for biogenesis end export of the 40S ribosomal subunit
CC independent on its methyltransferase activity (By similarity).
CC {ECO:0000250}.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC specifically methylates the N(7) position of a guanine in 18S rRNA.
CC Requires the methyltransferase adapter protein TRM112 for full rRNA
CC methyltransferase activity. Involved in the pre-rRNA processing steps
CC leading to small-subunit rRNA production independently of its RNA-
CC modifying catalytic activity. Important for biogenesis end export of
CC the 40S ribosomal subunit independent on its methyltransferase
CC activity. {ECO:0000250, ECO:0000250|UniProtKB:O43709}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a guanosine in 18S rRNA + S-adenosyl-L-methionine = an N(7)-
CC methylguanosine in 18S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:54584, Rhea:RHEA-COMP:13937, Rhea:RHEA-COMP:13938,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC ChEBI:CHEBI:74480; Evidence={ECO:0000250|UniProtKB:O43709};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43709}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:O43709}. Cytoplasm, perinuclear
CC region {ECO:0000250|UniProtKB:O43709}. Cytoplasm
CC {ECO:0000250|UniProtKB:O43709}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. BUD23/WBSCR22 family. {ECO:0000305}.
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DR EMBL; AAFI02000005; EAL72211.1; -; Genomic_DNA.
DR RefSeq; XP_646225.1; XM_641133.1.
DR AlphaFoldDB; Q55DA6; -.
DR SMR; Q55DA6; -.
DR EnsemblProtists; EAL72211; EAL72211; DDB_G0269722.
DR GeneID; 8617179; -.
DR KEGG; ddi:DDB_G0269722; -.
DR dictyBase; DDB_G0269722; -.
DR InParanoid; Q55DA6; -.
DR OMA; PHVWVGM; -.
DR PhylomeDB; Q55DA6; -.
DR Reactome; R-DDI-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR PRO; PR:Q55DA6; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016435; F:rRNA (guanine) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070476; P:rRNA (guanine-N7)-methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR039769; Bud23-like.
DR InterPro; IPR022238; Bud23_C.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12734; PTHR12734; 1.
DR Pfam; PF08241; Methyltransf_11; 1.
DR Pfam; PF12589; WBS_methylT; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Nucleus; Reference proteome; rRNA processing;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..287
FT /note="Probable 18S rRNA (guanine-N(7))-methyltransferase"
FT /id="PRO_0000330904"
FT REGION 214..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 249..287
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 287 AA; 32507 MW; F075024192E4B690 CRC64;
MSRPEHIAPP EIFYDDVESK KYSSNSRIIE IQTKMAERAY ELLAIPETAE GLMLLDIGCG
SGISGDVITD AGHYWIGCDI SQHMLDVAID REVEGDVMLR DIGQGFPFRA GSFDAAISIS
AIQWLCNAEK SHHNPRKRLH TFFQSLFNVL TRGGKAILQF YPENSAQIEM ITASALRCGF
SGGLLIDFPN SSKAKKYFLV LFTGNNNIMP SAKGVEGEEY EQQEEEDSNE VKYSNRKRDR
RRVTKSKGSA QHKTKEWIMN KKDRQRKQGR EIKNDSKFSG RKRGPKF
