ID   TREPH_PLEPU             Reviewed;         739 AA.
AC   A6YRN9;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Trehalose phosphorylase {ECO:0000312|EMBL:ABR88135.1};
DE            EC=2.4.1.231;
DE   AltName: Full=Trehalose synthase {ECO:0000250|UniProtKB:O75003};
DE            Short=TSase {ECO:0000250|UniProtKB:O75003};
DE   Flags: Precursor;
GN   Name=TP {ECO:0000250|UniProtKB:Q9UV63};
OS   Pleurotus pulmonarius (Indian oyster mushroom).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=28995;
RN   [1] {ECO:0000312|EMBL:ABR88135.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Tian Z.D., Xie C.H., Liu J.;
RT   "Isolation and functional characterization of trehalose phosphorylase gene
RT   in Pleurotus pulmonarius (Fr.).";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reversibly catalyzes the synthesis and degradation of
CC       trehalose from glucose and alpha-D-glucose 1-phosphate. The equilibrium
CC       lies in the direction of trehalose synthesis (By similarity).
CC       {ECO:0000250|UniProtKB:O75003, ECO:0000250|UniProtKB:Q9UV63}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-
CC         glucose 1-phosphate; Xref=Rhea:RHEA:16257, ChEBI:CHEBI:16551,
CC         ChEBI:CHEBI:17925, ChEBI:CHEBI:43474, ChEBI:CHEBI:58601;
CC         EC=2.4.1.231; Evidence={ECO:0000250|UniProtKB:Q9UV63};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:O75003}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EF645805; ABR88135.1; -; mRNA.
DR   AlphaFoldDB; A6YRN9; -.
DR   SMR; A6YRN9; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   BRENDA; 2.4.1.231; 4914.
DR   GO; GO:0033832; F:alpha,alpha-trehalose phosphorylase (configuration-retaining) activity; ISS:UniProtKB.
DR   GO; GO:0051156; P:glucose 6-phosphate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006006; P:glucose metabolic process; ISS:UniProtKB.
DR   GO; GO:0005991; P:trehalose metabolic process; ISS:UniProtKB.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glucose metabolism; Glycosyltransferase;
KW   Transferase.
FT   PROPEP          1..26
FT                   /evidence="ECO:0000250|UniProtKB:O75003"
FT                   /id="PRO_0000405295"
FT   CHAIN           27..739
FT                   /note="Trehalose phosphorylase"
FT                   /evidence="ECO:0000250|UniProtKB:O75003"
FT                   /id="PRO_0000405296"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   739 AA;  82028 MW;  6A8444D92D48EA95 CRC64;
     MSTPHHQFES KSSTAIRRRL SSSVSSKQRP NIMTTTFASL TPMWAGVAGT LVNNNTQYEI
     AVTVHDGVYS TDFASVIIPV TPGDTVKNSK DIEAQVLNLI RKFSAEHLCK FLGAGITLAL
     LKECPNLCTR LWLDMDIVPI VFNIKPFHTD SVTRPNIKHR ISSTTGSYVP SGSETPTVYV
     EASHLDDPSH LSPNAAQKLP IPRTLDEQSD SAARKCLMYF GPNNNPRLSI GARNQVTVDA
     GGKIHLIDDL EEYRETVGAG TWNAVIKLAD ELREKKVKIG FFSSTPQGGG VALMRHALIR
     FLTALDVDVA WYVPNPSPQV FRTTKNNHNI LQGVAAPDLR LTQEAKDAFD AWILKNGLRW
     TAEGGPLAPG GVDVVFIDDP QMPGLIPLIK KVRPEVPIVY RSHIEIRSDL VHVAGSPQEE
     VWKYLWNNIQ LADLFISHPV SKFVPSDVPT EKLALLGAAT DWLDGLNKDL DPWDSQFYMG
     EFRSPCAKEK MHELNWPARD YIVQVARFDP SKGIPNVVDS YYKFRNLLRT RSPDMDESEH
     PQLLICGHGA VDDPDASIIY DQIMALVNSD PYKEYAHDIV VMRLPPSDEL LNAMMANSRI
     ALQLSTREGF EVKVSEALHT GKPVIACRTG GIPLQIQHGK SGYLTTPGDN DAVAGHLYDL
     YTDEALYRKM SDFARTHVSD EVGTVGNAAA WLYLAVMYSR GEKIKPNGAW INDLLREETG
     EPYKEGETKL PRTKLDMQG