ID   TREY_ARTSQ              Reviewed;         775 AA.
AC   Q44315;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Maltooligosyl trehalose synthase;
DE            EC=5.4.99.15;
DE   AltName: Full=(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase;
GN   Name=treY;
OS   Arthrobacter sp. (strain Q36).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=104027;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8605217; DOI=10.1016/0304-4165(95)00139-5;
RA   Maruta K., Hattori K., Nakada T., Kubota M., Sugimoto T., Kurimoto M.;
RT   "Cloning and sequencing of trehalose biosynthesis genes from Arthrobacter
RT   sp. Q36.";
RL   Biochim. Biophys. Acta 1289:10-13(1996).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8611744; DOI=10.1271/bbb.59.2210;
RA   Nakada T., Maruta K., Tsusaki K., Kubota M., Chaen H., Sugimoto T.,
RA   Kurimoto M., Tsujisaka Y.;
RT   "Purification and properties of a novel enzyme, maltooligosyl trehalose
RT   synthase, from Arthrobacter sp. Q36.";
RL   Biosci. Biotechnol. Biochem. 59:2210-2214(1995).
CC   -!- FUNCTION: Catalyzes the conversion of maltooligosaccharide into the
CC       non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl
CC       alpha-D-glucoside) by intramolecular transglycosylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-[(1->4)-alpha-D-glucosyl](n-1)-D-glucose = 1-[(1->4)-alpha-
CC         D-glucosyl](n-1)-alpha-D-glucose; Xref=Rhea:RHEA:13621, Rhea:RHEA-
CC         COMP:14708, Rhea:RHEA-COMP:14709, ChEBI:CHEBI:140774,
CC         ChEBI:CHEBI:140775; EC=5.4.99.15;
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. {ECO:0000305}.
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DR   EMBL; D63343; BAA09667.1; -; Genomic_DNA.
DR   PIR; S65769; S65769.
DR   AlphaFoldDB; Q44315; -.
DR   SMR; Q44315; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; ag:BAA09667; -.
DR   BioCyc; MetaCyc:MON-6021; -.
DR   GO; GO:0047470; F:(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11336; AmyAc_MTSase; 1.
DR   Gene3D; 1.10.10.470; -; 1.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013797; Maltooligo_trehalose_synth_4.
DR   InterPro; IPR012767; Trehalose_TreY.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   TIGRFAMs; TIGR02401; trehalose_TreY; 1.
PE   1: Evidence at protein level;
KW   Isomerase.
FT   CHAIN           1..775
FT                   /note="Maltooligosyl trehalose synthase"
FT                   /id="PRO_0000054341"
SQ   SEQUENCE   775 AA;  85356 MW;  1BBD23024B2280FC CRC64;
     MRTPVSTYRL QIRKGFTLFD AAKTVPYLHS LGVDWVYLSP VLTAEQGSDH GYDVTDPSAV
     DPERGGPEGL AAVSKAARAA GMGVLIDIVP NHVGVATPAQ NPWWWSLLKE GRQSRYAEAF
     DVDWDLAGGR IRLPVLGSDD DLDQLEIRDG ELRYYDHRFP LAEGTYAEGD APRDVHARQH
     YELIGWRRAD NELNYRRFFA VNTLAGVRVE IPAVFDEAHQ EVVRWFREDL ADGLRIDHPD
     GLADPEGYLK RLREVTGGAY LLIEKILEPG EQLPASFECE GTTGYDALAD VDRVLVDPRG
     QEPLDRLDAS LRGGEPADYQ DMIRGTKRRI TDGILHSEIL RLARLVPGDA NVSIDAGADA
     LAEIIAAFPV YRTYLPEGAE VLKEACELAA RRRPELDQAI QALQPLLLDT DLELARRFQQ
     TSGMVMAKGV EDTAFFRYNR LGTLTEVGAD PTEFAVEPDE FHARLARRQA ELPLSMTTLS
     THDTKRSEDT RARISVISEV AGDWEKALNR LRDLAPLPDG PLSALLWQAI AGAWPASRER
     LQYYALKAAR EAGNSTNWTD PAPAFEEKLK AAVDAVFDNP AVQAEVEALV ELLEPYGASN
     SLAAKLVQLT MPGVPDVYQG TEFWDRSLTD PDNRRPFSFD DRRAALEQLD AGDLPASFTD
     ERTKLLVTSR ALRLRRDRPE LFTGYRPVLA SGPAAGHLLA FDRGTAAAPG ALTLATRLPY
     GLEQSGGWRD TAVELNTAMK DELTGAGFGP GAVKIADIFR SFPVALLVPQ TGGES